PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Laura Cerqueira (Author) Other; Ricardo M Fernandes (Author) Other; Rui M Ferreira (Author) Other; Fatima Carneiro (Author) FMUP; Mario Dinis Ribeiro (Author) FMUP; Ceu Figueiredo (Author) FMUP; Charles W Keevil (Author) Other; Nuno F Azevedo (Author) FEUP; Maria J Vieira (Author) Other

Journal

Title: BMC Microbiology

Vol. 11

Final page: 101

ISSN: 1471-2180

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 35 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-RG9

DOI: 10.1186/1471-2180-11-101

Abstract (EN): Background: Triple therapy is the gold standard treatment for Helicobacter pylori eradication from the human stomach, but increased resistance to clarithromycin became the main factor of treatment failure. Until now, fastidious culturing methods are generally the method of choice to assess resistance status. In this study, a new genotypic method to detect clarithromycin resistance in clinical samples, based on fluorescent in situ hybridization (FISH) using a set of peptide nucleic acid probes (PNA), is proposed. Results: The set of probes targeting the point mutations responsible for clarithromycin resistance was applied to H. pylori suspensions and showed 100% sensitivity and specificity (95% CI, 79.9-100 and 95% CI, 71.6-100 respectively). This method can also be amenable for application to gastric biopsy samples, as resistance to clarithromycin was also detected when histological slides were tested. Conclusions: The optimized PNA-FISH based diagnostic method to detect H. pylori clarithromycin resistance shown to be a very sensitive and specific method for the detection of clarithromycin resistance in the H. pylori smears and also proved to be a reliable method for the diagnosis of this pathogen in clinical samples and an alternative to existing plating methods.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7