Targeted Metabolite Analysis and Biological Activity of Pieris brassicae Fed with Brassica rapa var. rapa

• Type: Article in International Scientific Journal
• Date: 2009

Authors

David M Pereira (Author) Other; Alexandra Noites (Author) Other; Patricia Valentao (Author) FFUP; Federico Ferreres (Author) Other; Jose A Pereira (Author) Other; Luis Vale Silva (Author) Other; Eugenia Pinto (Author) FFUP; Paula B Andrade (Author) FFUP

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 57 No. 19

Pages: 483-489

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 15 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-003-NQJ

DOI: 10.1021/jf8031638

Resumo (PT): For the first time, an insect−plant system, Pieris brassicae fed with Brassica rapa var. rapa, was tested for its biological capacity, namely, antioxidant (DPPH•, •NO, and O2•− radicals) and antimicrobial (bacteria and fungi) activities. Samples from the insect’s life cycle (larvae, excrements, exuviae, and butterfly) were always found to be more efficient than the host plant. Also, P. brassicae materials, as well as its host plant, were screened for phenolics and organic acids. The host plant revealed higher amounts of both compounds. Two phenolic acids, ferulic and sinapic, as well as kaempferol 3-O-sophoroside, were common to insect (larvae and excrements) and plant materials, with excrements being considerably richer. Detection of sulfated compounds in excrements, absent in host plant, revealed that metabolic processes in this species involved sulfation. Additionally, deacylation and deglycosilation were observed. All matrices presented the same organic acids qualitative profile, with the exception of excrements <br> <br> <a target="_blank" href="http://pubs.acs.org/doi/abs/10.1021/jf8031638"> Texto integral </a> <br> <br>

Abstract (EN): For the first time, an insect-plant system, Pieris brassicae fed with Brassica rapa var. rapa, was tested for its biological capacity, namely, antioxidant (DPPH(center dot), (center dot)NO, and O(2)(center dot-) radicals) and antimicrobial (bacteria and fungi) activities. Samples from the insect's life cycle (larvae, excrements, exuviae, and butterfly) were always found to be more efficient than the host plant. Also, P. brassicae materials, as well as its host plant, were screened for phenolics and organic acids. The host plant revealed higher amounts of both compounds. Two phenolic acids, ferulic and sinapic, as well as kaempferol 3-O-sophoroside, were common to insect (larvae and excrements) and plant materials, with excrements being considerably richer. Detection of sulfated compounds in excrements, absent in host plant, revealed that metabolic processes in this species involved sulfation. Additionally, deacylation and deglycosilation were observed. All matrices presented,the same organic acids qualitative profile, with the exception of excrements.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7