Cultivation and functional characterization of 79 planctomycetes uncovers their unique biology

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Wiegand, S (Author) Other; Jogler, M (Author) Other; Boedeker, C (Author) Other; Pinto, D (Author) Other; Vollmers, J (Author) Other; Rivas Marin, E (Author) Other; Kohn, T (Author) Other; Peeters, SH (Author) Other; Heuer, A (Author) Other; Rast, P (Author) Other; Oberbeckmann, S (Author) Other; Bunk, B (Author) Other; Jeske, O (Author) Other; Meyerdierks, A (Author) Other; Storesund, JE (Author) Other; Kallscheuer, N (Author) Other; Lucker, S (Author) Other; lage, om (Author) FCUP; Pohl, T (Author) Other; Merkel, BJ (Author) Other; Hornburger, P (Author) Other; Mueller, RW (Author) Other; Bruemmer, F (Author) Other; Labrenz, M (Author) Other; Spormann, AM (Author) Other; Op den Camp, HJM (Author) Other; Overmann, J (Author) Other; Amann, R (Author) Other; Jetten, MSM (Author) Other; Mascher, T (Author) Other; Medema, MH (Author) Other; Devos, DP (Author) Other; Kaster, AK (Author) Other; Ovreas, L (Author) Other; Rohde, M (Author) Other; Galperin, MY (Author) Other; Jogler, C (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: NATURE MICROBIOLOGY

Vol. 5

Pages: 126-140

ISSN: 2058-5276

Indexing

ISI Web of Knowledge - 122 Citations

Scopus - 120 Citations

Other information

Authenticus ID: P-00R-DQ8

DOI: 10.1038/s41564-019-0588-1

Abstract (EN): When it comes to the discovery and analysis of yet uncharted bacterial traits, pure cultures are essential as only these allow detailed morphological and physiological characterization as well as genetic manipulation. However, microbiologists are struggling to isolate and maintain the majority of bacterial strains, as mimicking their native environmental niches adequately can be a challenging task. Here, we report the diversity-driven cultivation, characterization and genome sequencing of 79 bacterial strains from all major taxonomic clades of the conspicuous bacterial phylum Planctomycetes. The samples were derived from different aquatic environments but close relatives could be isolated from geographically distinct regions and structurally diverse habitats, implying that 'everything is everywhere'. With the discovery of lateral budding in 'Kolteria novifilia' and the capability of the members of the Saltatorellus clade to divide by binary fission as well as budding, we identified previously unknown modes of bacterial cell division. Alongside unobserved aspects of cell signalling and small-molecule production, our findings demonstrate that exploration beyond the well-established model organisms has the potential to increase our knowledge of bacterial diversity. We illustrate how 'microbial dark matter' can be accessed by cultivation techniques, expanding the organismic background for small-molecule research and drug-target detection.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15