Histological Evidence of Wound Healing Improvement in Rats Treated with Oral Administration of Hydroalcoholic Extract of Vitis labrusca

• Type: Another Publication in an International Scientific Journal
• Date: 2021

Authors

Santos, TS (Author) Other; dos Santos, IDD (Author) Other; Pereira, RN (Author) Other; Gomes, SVF (Author) Other; Lima Verde, IB (Author) Other; Marques, MN (Author) Other; Cardoso, JC (Author) Other; Severino, P (Author) Other; Souto, EB (Author) Other; de Albuquerque, RLC (Author) Other

Journal

Title: Current Issues in Molecular Biology

Vol. 43

Pages: 335-352

ISSN: 1467-3037

Indexing

ISI Web of Knowledge - 0 Citations

Scientific classification

CORDIS: Health sciences

Other information

Authenticus ID: P-00V-460

DOI: 10.3390/cimb43010028

Abstract (EN): Plant extracts rich in phenolic compounds have been demonstrated to accelerate wound healing, but their use by oral route has been poorly studied. The leaves of Vitis labrusca are rich in phenolic acids and flavonoids. The goal of this study was to assess the healing properties of the oral administration of hydroalcoholic extract of V labrusca leaves (HEVL) in a murine model. HEVL was obtained by Soxhlet and dynamic maceration, and their yield and phenolic acids and flavonoid contents were determined. For the wound healing assay, 8 mm wounds were performed on the back of 48 Wistar rats, assigned into four groups (n = 12): CTR (distilled water), HEVL100, HEVL200, and HEVL300 (HEVL at 100, 200, and 300 mg/kg, respectively). On days 7 and 14, wound closure rates were assessed, and the healing wounds were subjected to histological analysis. Soxhlet-obtained extract was selected for the wound healing assay because it provided a higher yield and phenolic acid and flavonoid contents. HEVL significantly reduced leukocytosis in the peripheral blood (p < 0.05), accelerated wound closure (p < 0.05), and improved collagenization (p < 0.05) on day 7, as well as enhanced the epidermal tissue thickness (p < 0.001) and elastic fiber deposition on day 14 (p < 0.01). Furthermore, HEVL promoted an increase in the histological grading of wound healing on both days 7 and 14 (p < 0.01). The doses of 200 and 300 mg/kg provided better results than 100 mg/Kg. Our data provide histological evidence that the oral administration of HEVL improves wound healing in rodents. Therefore, the extract can be a potential oral medicine for healing purposes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 18