Cell-free DNA as a marker for the outcome of end-stage renal disease patients on haemodialysis

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Coimbra, S (Author) Other; Rocha, S (Author) Other; Nascimento, H (Author) Other; Valente, MJ (Author) Other; Cristina Catarino (Author) FFUP; Rocha Pereira, P (Author) Other; Sameiro Faria, M (Author) Other; José Oliveira (Author) FMUP; Madureira, J (Author) Other; Fernandes, JC (Author) Other; Miranda, V (Author) Other; Belo, L (Author) FFUP; Bronze da Rocha, E (Author) FFUP; Alice Santos Silva (Author) FFUP

Journal

Title: CKJ: Clinical Kidney Journal

Vol. 14

Pages: 1371-1378

ISSN: 2048-8505

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00S-XGA

DOI: 10.1093/ckj/sfaa115

Abstract (EN): Background. DNA damage and inflammation are common in end-stage renal disease (ESRD). Our aim was to evaluate the levels of circulating cell-free DNA (cfDNA) and the relationship with inflammation, anaemia, oxidative stress and haemostatic disturbances in ESRD patients on dialysis. By performing a 1-year follow-up study, we also aimed to evaluate the predictive value of cfDNA for the outcome of ESRD patients. Methods. A total of 289 ESRD patients on dialysis were enrolled in the study: we evaluated cfDNA, haemogram, serum iron, hepcidin, inflammatory and oxidative stress markers, and haemostasis. Events and causes of deaths were recorded throughout the follow-up period. Results. ESRD patients, as compared with controls, presented significantly higher levels of cfDNA, hepcidin, and inflammatory and oxidative stress markers, and significantly lower values of iron and anaemia-related haemogram parameters. The all-cause mortality rate was 9.7%; compared with alive patients, deceased patients (n = 28) were older and presented significantly higher values of inflammatory markers and of cfDNA, which was almost 2-fold higher. Furthermore, cfDNA was the best predictor of all-cause mortality and cardiovascular mortality in ESRD patients, in both unadjusted and adjusted models for basic confounding factors in dialysis. Conclusions. Our data show cfDNA to be a valuable predictive marker of prognosis in ESRD patients on dialysis treatment; high levels of cfDNA were associated with a poor outcome.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8