Neural cell growth on TiO2 anatase nanostructured surfaces

• Type: Article in International Scientific Journal
• Date: 2009

Authors

J.E.Collazos-Castro (Author) Other; Ana M.Cruz (Author) Other; Mónica Carballo-Vila (Author) Other; Mónica Lira-Cantu (Author) Other; Llibertat Abad (Author) Other; Ángel Pérez del Pino (Author) Other; Jordi Fraxedas (Author) Other; Aurélie San Juan (Author) Other; Carlos Fonseca (Author) FEUP; Ana P. Pêgo (Author) Other; N.Casan-Pastor (Author) Other; Nieves Casañ-Pastor (Adapter) Other

Journal

Title: Thin Solid Films

Pages: 160-170

ISSN: 0040-6090

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 20 Citations

ISI Web of Science

Scopus - 23 Citations

INSPEC

Scientific classification

FOS: Engineering and technology > Other engineering and technologies

CORDIS: Technological sciences

Other information

Authenticus ID: P-003-EE5

DOI: 10.1016/j.tsf.2009.06.048

Abstract (EN): Titanium oxides have anti-inflammatory activity and tunable electrochemical properties that make them attractive materials for biomedical applications. This work investigated the compatibility of nanometric coatings of low-temperature phases of TiO2 with neurons in 4-day and 10-day cultures, using different cell densities to quantify cell survival and neurite extension. TiO2 films were prepared by sol-gel and thermal treatment (250-450 °C) of hydrolyzed titanium tetra-isopropoxide on electrically conducting or insulating slides. The conducting substrates were not passivated by the nanometric oxide layer and could be used as electrodes. Characterization of the films showed nano-structured TiO2 containing exclusively Ti+4 valence in anatase and amorphous phases. When coated with polylysine, all films permitted good neuron attachment and survival for at least ten days in culture. However, they generally reduced neurite growth compared to cell culture borosilicate glass, with dendrites more affected than axons. The analyses of surface topography, hydrophilicity, charge and chemical composition revealed that TiO2 chemistry was the main factor responsible for neurite inhibition

Language: English

Type (Professor's evaluation): Scientific