A Novel Approach for Monitoring the Volatile Metabolome in Biological Samples from Ruminants through Miniaturized Liquid-Liquid Extraction and Multiclass Gas Chromatography Analysis

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Cordeiro, L (Author) Other; Cabrita, ARJ (Author) Other; Oliveira, HM (Author) Other; Maia, MRG (Author) ICBAS; Rodrigues, JA (Author) FCUP; Antonio J M Fonseca (Author) ICBAS; Valente, IM (Author) FCUP

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 70

Pages: 3886-3897

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-00W-CMN

DOI: 10.1021/acs.jafc.1c06662

Abstract (EN): A straightforward and versatile methodology for the extraction of volatile metabolites in biological samples from ruminants for gas chromatography analysis is proposed. The methodology was applied in the determination of multiclass metabolites (short-chain fatty acids, aldehydes, alcohols, ketones, esters, phenols, and sulfides) in different analytical matrices (rumen fluid, urine, and feces) collected from Holstein cows. The 24 multiclass volatile metabolites reported in the different biological samples and their respective concentrations were critically discussed in the context of digestive physiology. Most detected compounds are derived from the rumen and lower gut fermentation of carbohydrates, proteins, and lipids or their metabolism, being consistent with the prior state of the art. The proposed method also takes advantage of the already existing tools in animal nutrition laboratories, providing a novel methodological ground that can generate relevant bioanalytical information with a significant impact on ruminant's nutritional studies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12