Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Sandra G. Silva (Author) FCUP; Pinheiro, M (Author) Other; Pereira, R (Author) Other; Dias, AR (Author) Other; Ferraz, R (Author) Other; Prudencio, C (Author) Other; Eaton, PJ (Author) Other; Salette Reis (Author) FFUP; M. Luisa C. do Vale (Author) FCUP

Journal

Title: Biochimica et Biophysica Acta: Biomembranes

Vol. 1864

ISSN: 0005-2736

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 1 Citation

Other information

Authenticus ID: P-00W-MGY

DOI: 10.1016/j.bbamem.2022.183969

Abstract (EN): The antimicrobial activity of two serine derived gemini cationic surfactants, amide (12Ser)2CON12 and ester (12Ser)2COO12, was tested using sensitive, E. coli ATCC 25922 and S. aureus ATCC 6538, and resistant, E. coli CTX M2, E. coli TEM CTX M9 and S. aureus ATCC 6538 and S. aureus MRSA ATCC 43300 Gram-positive and Gram-negative bacteria strains. Very low MIC values (5 mu M) were found for the two resistant strains E.coli TEM CTX M9 and S. aureus MRSA ATCC 43300, in the case of the amide derivative, and for S. aureus MRSA ATCC 43300, in the case of the ester derivative. The interaction of the serine amphiphiles with lipid-model membranes (DPPG and DPPC) was investigated using Langmuir monolayers. A more pronounced effect on the DPPG than on the DPPC monolayer was observed. The effect induced by the surfactants on bacteria membrane was explored by Atomic Force Microscopy. A clear disruption of the bacteria membrane was observed for E. coli TEM CTX M9 upon treatment with (12ser)2CON12, whereas for the S. aureus MRSA few observable changes in cell morphology were found after treatment with either of the two surfactants. The cytotoxicity of the two compounds was assessed by hemolysis assay on human red blood cells (RBC). The compounds were shown to be non-cytotoxic up to 10 mu M. Overall, the results reveal a promising potential, in particular of the amide derivative, as antimicrobial agent for two strains of antibiotic resistant bacteria.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10