Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Maria Judite Barbosa (Author) FEUP; Mário A. Barbosa (Author) FEUP; Artur Perez Águas (Author) FEUP

Journal

Title: Biomaterials

Vol. 25 No. 13

Pages: 2557-2563

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Science

Scientific classification

FOS: Medical and Health sciences > Medical biotechnology

CORDIS: Technological sciences > Engineering > Biomaterial engineering

Other information

DOI: 10.1016/j.biomaterials.2003.09.047

Abstract (EN): The acute inflammatory response and the adhesion of cells to self-assembled monolayers (SAMs) of well-defined surface chemistry was studied in vivo using a rodent air-pouch model of inflammation. SAMs with three different terminal functional groups (OH, COOH and CH3) were implanted in subcutaneous air pouches induced in BALB/c mice. After 24 h, inflammatory cells were recovered from the air pouches and the implants were removed and prepared for observation by scanning electron microscopy (SEM). The implants coated with OH and CH3, were found to cause the highest recruitment of inflammatory cells into the subcutaneous pouches. Polymorphonuclear neutrophils (PMNs) leukocytes predominated over mononuclear cells in inflammatory exudates of SAMs-coated implants, the opposite being found in uncoated implants (controls). CH3-coated implants induced the highest number of inflammatory cells and also the largest percentage of PMNs seen in the subcutaneous pouches. Control and OH-covered implants presented the higher densities of attached inflammatory cells detected by SEM. In contrast, the CH3-coated implants showed a very low density of cells adherent to the implant surface. We conclude that the chemical nature and the degree of hydrophobicity of the surface of implants modulate both the local acute inflammatory reaction and the adhesion of leukocytes. (C) 2003 Elsevier Ltd. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7