Effect of skimmed milk on intestinal tract: Prevention of increased reactive oxygen species and nitric oxide formation

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Pinho, SCM (Author) Other; Faria, MA (Author) Other; Melo, A (Author) Other; Pinto, E (Author) Other; Agostinho Almeida (Author) FFUP; Alves, R (Author) Other; A. Cabrita (Author) ICBAS; Antonio J M Fonseca (Author) ICBAS; Ferreira, Isabel (Author) FFUP

Journal

Title: International Dairy Journal

Vol. 118

ISSN: 0958-6946

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00T-K69

DOI: 10.1016/j.idairyj.2021.105046

Abstract (EN): The capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2O2) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopoly-saccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2O2-induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9