Oxidative stress response in the adult rat retina and plasma after repeated administration of methamphetamine

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Melo, P (Author) Other; Zanon Moreno, V (Author) Other; Alves, CJ (Author) Other; Ana Magalhaes (Author) Other; tavares, ma (Author) FMUP; Dolores Pinazo Duran, MD (Author) Other; Pedro Moradas Ferreira (Author) ICBAS

Journal

Title: Neurochemistry International

Vol. 56

Pages: 431-436

ISSN: 0197-0186

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 24 Citations

Other information

Authenticus ID: P-003-9HK

DOI: 10.1016/j.neuint.2009.11.017

Abstract (EN): Methamphetamine (MA) is a psychostimulant that target the sensory systems, with the neurosensory retina having been shown to be affected. in the brain, MA-related toxicity can be linked to oxidative stress; the same relationship has yet to be established for the retina. The aim of this study, therefore, was to evaluate the effects of repeated exposure to MA on oxidative stress parameters in the rat retina. Oxidative stress parameters in the blood plasma were also assessed. Male Wistar rats were given 5 mg/kg MA every 2 h for a period of 6 h (i.e., 4 injections) daily between postnatal day (PND) 91 and 100. Evolution of body weight was registered. Rats were sacrificed at PND 110. Blood plasma was collected and immediately frozen for storage at -70 degrees C. The eyes were enucleated, and the retina and choroids rapidly dissected on ice under dim light also to be stored at -70 degrees C. Lipid peroxidation activity was measured by the thiobarbituric acid (TBA) test. Total antioxidant status, superoxide dismutase (SOD) activity, catalase (Cat) activity, and nitrogen oxides contents were also determined. Lipid peroxidation was significantly higher in the retina and blood plasma of the MA-treated rats. Total antioxidant levels were significantly lower in both retina and blood plasma of the MA-treated rats. The activity of SOD was significantly increased in the retina and blood plasma of MA-treated rats. Catalase activity did not differ between groups in either the retina or the blood plasma. Nitric oxide production was significantly higher in both the retina and blood plasma in the MA-treated animals. The overall findings show that the oxidative stress defence mechanisms in the retina are compromised by MA toxicity. The results are similar to those found in the brain, and, moreover, showed some correlation with the blood plasma.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6