DOPAMINE FORMATION, FROM ITS IMMEDIATE PRECURSOR 3,4-DIHYDROXYPHENYLALANINE, ALONG THE RAT DIGESTIVE-TRACT

• Type: Article in International Scientific Journal
• Date: 1993

Authors

vieira-coelho, ma (Author) Other; SOARESDASILVA, P (Author) Other

Journal

Title: Fundamental and Clinical Pharmacology

Vol. 7

Pages: 235-243

ISSN: 0767-3981

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 50 Citations

Other information

Authenticus ID: P-001-NK5

DOI: 10.1111/j.1472-8206.1993.tb00237.x

Abstract (EN): The formation of dopamine, from L-3,4-dihydroxyphenylalanine (L-dopa), in fragments of non-glandular and glandular stomach, duodenum, jejunum, ileum and proximal and distal colon of the rat was examined. The deamination of newly-formed dopamine into 3,4-dihydroxyphenylacetic acid (dopac) was also studied. The synthesis of dopamine in tissues incubated with 500 muM L-dopa for 20 min in conditions of catechol-O-methyltransferase (Comt) inhibition was found to be in the duodenum, jejunum and ileum 2-fold that in the proximal colon, 6-fold that in the glandular stomach and 120-fold that in the non-glandular stomach and distal colon. The formation of dopac in these tissues followed the pattern of amine formation. In the jejunum, the formation of dopamine and dopac was found to be dependent on the concentration of L-dopa (50 to 5000 muM) used. In another set of experiments, it was found that the formation of dopamine in jejunal segments loaded with increasing concentrations of L-dopa (50, 100 and 500 muM) was a time-dependent process. The rate constant (k) of formation of dopamine as a function of time was found to be similar (0.050 +/- 0.005) with either concentration of L-dopa; the rate constant of dopac formation in these experiments was, in contrast, found to be greater at the highest concentrations of L-dopa (100 and 500 muM). Aromatic L-amino acid decarboxylase (Aaad) activity determined in homogenates of the jejunal mucosa was found to be twice that observed in homogenates of the remaining jejunal wall (muscular). The V(max) (nmol/g/h) and K(m) (muM) of Aaad was, respectively, in the jejunal mucoase 1324 +/- 206 and 124 +/- 13 and in the jejunal muscular wall 680 +/- 3 5 and 136 +/- 25. The results presented here show that the formation of dopamine along the digestive tract of the rat presents a heterogeneous pattern, the small intestine and the proximal colon being the areas where this reaction appears to be more important.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9