Development of a molecular imprinted electrochemiluminescence sensor for amitriptyline detection: From MD simulations to experimental implementation

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Rebelo, P (Author) Other; Pacheco, JG (Author) Other; Voroshylova, IV (Author) Other; Natalia N D S Cordeiro (Author) FCUP; Delerue Matos, C (Author) Other

Journal

Title: Electrochimica Acta

Vol. 397

ISSN: 0013-4686

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-00V-FWJ

DOI: 10.1016/j.electacta.2021.139273

Abstract (EN): Amitriptyline (AMT) frequent presence in environmental waters reflects the continuous consumption growth and raises issues on the importance of its monitorization. In this work, a sensitive and selective electrochemiluminescence (ECL) sensor was constructed using molecularly imprinted polymer (MIP) recognition element for AMT detection. Molecular dynamics (MD) simulations were performed to select the best functional monomer. Precipitation polymerization was followed to prepare the MIP micro spheres using methacrylic acid (MAA) as functional monomer, ethylene glycol methacrylate (EGDMA) as crosslinker and chloroform (CHL) as solvent. The MIP sensor was then prepared on a low cost and disposable screen-printed electrodes (SPCEs), previously modified with single-walled carbon nanotubes (SWCNTs), by drop coating a solution containing the MIP microspheres synthesized. The mechanism of detection was based in the system Ru(bpy)(3)(2+)/AMT, where AMT acts as co-reactor of Ru(bpy)(3)(2+) ECL. Several parameters controlling the preparation process of the sensor and AMT detection were optimised. The MIP/SWCNTs/SPCE ECL sensor showed good analytical performance with a linear correlation between ECL signal and the AMT concentration ranging from 0.1 to 200 mu M (R-2 = 0.9991). The limits of detection (LOD) and quantification (LOQ) were found to be 0.4 mu M (S/N = 3) and 1.5 mu M (S/N = 10), respectively. The MIP ECL sensor displayed good selectivity to recognise AMT molecules when compared with analoge structures and it was successfully applied in real water samples with good recovery values (90 to 112%). The developed MIP ECL sensor is suitable for integration with portable devices for AMT detection in environmental waters.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11