Targeted deletion of a 170-kb cluster of LINE-1 repeats and implications for regional control

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Soares, ML (Author) FMUP; Edwards, CA (Author) Other; Dearden, FL (Author) Other; Ferron, SR (Author) Other; Curran, S (Author) Other; Corish, JA (Author) Other; Rancourt, RC (Author) Other; Allen, SE (Author) Other; Charalambous, M (Author) Other; Ferguson Smith, MA (Author) Other; Rens, W (Author) Other; Adams, DJ (Author) Other; Ferguson Smith, AC (Author) Other

Journal

Title: Genome Research

Vol. 28

Pages: 345-356

ISSN: 1088-9051

Publisher: Cold Spring Harbor Laboratory Press

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00N-RD9

DOI: 10.1101/gr.221366.117

Abstract (EN): Approximately half the mammalian genome is composed of repetitive sequences, and accumulating evidence suggests that some may have an impact on genome function. Here, we characterized a large array class of repeats of long-interspersed elements (LINE-1). Although widely distributed in mammals, locations of such arrays are species specific. Using targeted deletion, we asked whether a 170-kb LINE-1 array located at a mouse imprinted domain might function as a modulator of local transcriptional control. The LINE-1 array is lamina associated in differentiated ES cells consistent with its AT-richness, and although imprinting occurs both proximally and distally to the array, active LINE-1 transcripts within the tract are biallelically expressed. Upon deletion of the array, no perturbation of imprinting was observed, and abnormal phenotypes were not detected in maternal or paternal heterozygous or homozygous mutant mice. The array does not shield nonimprinted genes in the vicinity from local imprinting control. Reduced neural expression of protein-coding genes observed upon paternal transmission of the deletion is likely due to the removal of a brain-specific enhancer embedded within the LINE array. Our findings suggest that presence of a 170-kb LINE-1 array reflects the tolerance of the site for repeat insertion rather than an important genomic function in normal development.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12