Effects of gold nanoparticles in gilthead seabream-A proteomic approach

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Barreto, A (Author) Other; Carvalho, A (Author) Other; Campos, A (Author) Other; Osorio, H (Author) Other; Pinto, E (Author) Other; Agostinho Almeida (Author) FFUP; Trindade, T (Author) Other; Soares, AMVM (Author) Other; Hylland, K (Author) Other; Loureiro, S (Author) Other; Oliveira, M (Author) Other

Journal

Title: Aquatic Toxicology

Vol. 221

ISSN: 0166-445X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00R-R15

DOI: 10.1016/j.aquatox.2020.105445

Abstract (EN): Despite the widespread use of nanoparticles (NPs), there are still major gaps of knowledge regarding the impact of nanomaterials in the environment and aquatic animals. The present work aimed to study the effects of 7 and 40 nm gold nanoparticles (AuNPs) - citrate and polyvinylpyrrolidone (PVP) coated - on the liver proteome of the estuarine/marine fish gilthead seabream (Sparus aurata). After 96 h, exposure to AuNP elicited alterations on the abundance of 26 proteins, when compared to the control group. AuNPs differentially affected several metabolic pathways in S. aurata liver cells. Among the affected proteins were those related to cytoskeleton and cell structure, gluconeogenesis, amino acids metabolism and several processes related to protein activity (protein synthesis, catabolism, folding and transport). The increased abundance of proteins associated with energy metabolism (ATP synthase subunit beta), stress response (94 kDa glucose-regulated protein) and cytoskeleton structure (actins and tubulins) may represent the first signs of cellular oxidative stress induced by AuNPs. Although higher gold accumulation was found in the liver of S. aurata exposed to 7 nm PVP-AuNPs, the 7 nm cAuNPs were more bioactive, inducing more effects in liver proteome. Gold accumulated more in the spleen than in the other assessed tissues of S. aurata exposed to AuNPs, highlighting its potential role on the elimination of these NPs.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12