Development and validation of a gas chromatography mass spectrometry method for the analysis of phytoestrogens, phytosterols and mycotoxins in estuarine water samples

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Ribeiro, C (Author) Other; Tiritan M.E. (Author) FFUP

Journal

Title: International Journal of Environmental Analytical Chemistry

Vol. 95

Pages: 187-202

ISSN: 0306-7319

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00A-8ES

DOI: 10.1080/03067319.2014.1002492

Abstract (EN): Many pollutants currently released into the environment may cause adverse effects on exposed organisms leading to disruption of the endocrine system. Among them are natural compounds such as phytoestrogens and phytosterols, found in various plants and mycotoxins produced by a wide range of fungal species. The presence of some classes of phytoestrogens and phytosterols has been demonstrated in environmental samples while little information can be found about the presence of mycotoxins. Due to the complexity of environmental matrices and the low concentrations normally found, sensitive and selective methods are required for an unequivocal quantification of this type of compounds. The aim of this study was to develop and validate an analytical method for the simultaneous quantification of seven phytoestrogens, two phytosterols and three mycotoxins in estuarine water samples. The method consisted of the preconcentration of estuarine water samples (1L) on 200mg OASIS HLB cartridges, followed by a clean-up step in 1g silica cartridges and quantification by gas chromatography with mass spectrometry ion trap analyser. Validation parameters were evaluated and the method demonstrated to be selective and linear with correlations higher than 0.99. Method detection limits were in the ng L-1 levels ranging from 18.0 to 90.0ngL(-1), recovery rates ranged from 59.8% to 99.5% and precision (RSD) from 5.5% and 9.7%. The feasibility of the method was demonstrated in surface water samples from Douro River estuary, a polluted estuary, with reported incidence of endocrine-disrupting phenomena.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16