Optimization of high pressure bioactive compounds extraction from pansies (Viola x wittrockiana) by response surface methodology

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Fernandes, L (Author) Other; Susana Casal (Author) FFUP; Pereira, JA (Author) Other; Ramalhosa, E (Author) Other; Saraiva, JA (Author) Other

Journal

Title: High Pressure Research

Vol. 37

Pages: 415-429

ISSN: 0895-7959

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 16 Citations

Other information

Authenticus ID: P-00M-X12

DOI: 10.1080/08957959.2017.1347925

Abstract (EN): Response surface methodology (RSM) was employed for the first time to optimize high pressure extraction (HPE) conditions of bioactive compounds from pansies, namely: pressure (X-1: 0-500 MPa), time (X-2: 5-15 min) and ethanol concentration (X-3: 0-100%). Consistent fittings using second-order polynomial models were obtained for flavonoids, tannins, anthocyanins, total reducing capacity (TRC) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The optimum extraction conditions based on combination responses for TRC, tannins and anthocyanins were: X-1=384 MPa, X-2=15 min and X-3=35% (v/v) ethanol, shortening the extraction time when compared to the classic method of stirring (approx. 24h). When the optimum extraction conditions were applied, 65.1 mg of TRC, 42.8 mg of tannins and 56.15 mg of anthocyanins/g dried flower were obtained. Thus, HPE has shown to be a promising technique to extract bioactive compounds from pansies, by reducing the extraction time and by using green solvents (ethanol and water), for application in diverse industrial fields.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15