Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Pinto, G (Author) Other; Valentim, H (Author) Other; Costa, A (Author) Other; Castro, S (Author) Other; Santos, C (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Vol. 38

Pages: 569-572

ISSN: 1054-5476

Publisher: Springer

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 35 Citations

Other information

Authenticus ID: P-000-MHM

DOI: 10.1079/ivp2002352

Abstract (EN): Somatic embryos were obtained from a 60-yr-old Quercus suber L. tree. Leaf explants were cultivated on Murashige and Skoog medium with 30 gl(-1) sucrose, 3 gl(-1) gelrite, pH adjusted to 5.8, and different growth regulator combinations. Callus induction took place at 24 +/- 1degreesC in the dark during the first 3 wk. After 3 mo. calluses that showed embryogenic structures were transferred to the same medium without growth regulators. Somatic embryogenesis was only observed in calluses induced on E3 medium (supplemented with 4.5 muM 2,4-dichlorophenoxyacetic acid and 9.0 muM zeatin). On average, 7.5% of the initial explants formed embryogenic calluses in this medium. Somatic embryo proliferation was high due to secondary embryogenesis. On average, 10% of the somatic embryos germinated and 40% of these germinated embryos converted into plants. Plants were elongated on the same medium without growth regulators and acclimated to greenhouse conditions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4