Pseudomonas grimontii biofilm protects food contact surfaces from Escherichia coli colonization

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Luciana Gomes (Author) FEUP; Jean-Christophe Piard (Author) Other; Romain Briandet (Author) Other; Filipe Mergulhão (Author) FEUP

Journal

Title: LWT - Food Science and Technology

Vol. 85 No. Part B: SI

Pages: 309-315

ISSN: 0023-6438

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

ISI Web of Science

Scopus - 16 Citations

Associated Projects

SUSCLEAN - Sustainable Cleaning and Disinfection in Fresh-Cut Food Industries

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

Other information

Authenticus ID: P-00N-573

DOI: 10.1016/j.lwt.2017.03.005

Abstract (EN): Escherichia coli typically colonizes food contact surfaces in the presence of other bacterial strains. The aim of this work was to evaluate the influence of a resident strain isolated from a fresh-cut salad industry (Pseudomonas grimontii 13A10) on the development of a model pathogen (E. coli) on bare stainless steel (SST) and stainless steel coated with diamond-like carbon (DLC) films, a-C:H:Si:0 designated by SICON (R) and a-C:H:Si designated by SICAN. The bacterial composition and spatial organization of single- and dual-species biofilms were analyzed by confocal laser scanning microscopy (CLSM). Biofilms were developed for 1 and 3 days at 10 degrees C and it was observed that the biovolume of E. coli biofilms in the presence of P. grimontii was lower than in axenic conditions, suggesting that the isolate can protect food contact surfaces from pathogen colonization. After 3 days, the dual-species biofilms contained essentially P. grimontii cells and no preferential vertical distribution of bacterial strains was observed. The use of a-C:H:Si:O coated surfaces reduced the short-term colonization of the model pathogen in single- and dual species biofilms, whereas decreased colonization by the non-pathogenic strain was only observed after 3 days. (C) 2017 Elsevier Ltd. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7