Synergism between A(2A)-adenosine receptor activation and vasoactive intestinal peptide to facilitate [H-3]-acetylcholine release from the rat motor nerve terminals

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Paulo Correia de Sá (Author) ICBAS; Timoteo, MA (Author) Other; Ribeiro, JA (Author) Other

Journal

Title: Neuroscience Letters

Vol. 309

Pages: 101-104

ISSN: 0304-3940

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-000-TKE

DOI: 10.1016/s0304-3940(01)02030-4

Abstract (EN): The effect of vasoactive intestinal peptide (VIP) on evoked [H-3]-acetylcholine ([H-3]-ACh) release from motor nerve terminals, and its interaction with presynaptic facilitatory A(2A)-adenosine receptors was investigated in the rat phrenic nerve-hemidiaphragms. Facilitation of [H-3]-ACh release by VIP (100 nM) only becomes apparent when high frequency (50 Hz) or long lasting pulses (1 ms) were delivered to the phrenic nerve; VIP excitation was prevented by removal of endogenous adenosine tonus, with adenosine deaminase (2.5 units/ml) or with the A(2A)-receptor antagonist, 3,7-dimethyl-1-propargyl xanthine, (10 muM). Pretreatment with the selective A(2A)-receptor agonist, CGS 21680C (2 nM), potentiated the neurofacilitatory action of VIP (100 nM). The results suggest that tonic A(2A)-receptors activation by endogenous adenosine is required to trigger the facilitatory action of VIP on evoked [H-3]-ACh release from motor nerve endings.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4