Human autoantibodies reveal titin as a chromosomal protein

• Type: Another Publication in an International Scientific Journal
• Date: 1998

Authors

Machado, C (Author) Other; Sunkel, CE (Author) ICBAS; Andrew, DJ (Author) Other

Journal

Title: Journal of Cell Biology

Vol. 141

Pages: 321-333

ISSN: 0021-9525

Publisher: Rockefeller University Press

Indexing

ISI Web of Knowledge - 97 Citations

Scopus - 104 Citations

Other information

Authenticus ID: P-001-83A

DOI: 10.1083/jcb.141.2.321

Abstract (EN): Assembly of the higher-order structure of mitotic chromosomes is a prerequisite for proper chromosome condensation, segregation and integrity. Understanding the details of this process has been limited because very few proteins involved in the assembly of chromosome structure have been discovered. Using a human autoimmune scleroderma serum that identifies a chromosomal protein in human cells and Drosophila embryos, we cloned the corresponding Drosophila gene that encodes the homologue of vertebrate titin based on protein size, sequence similarity, developmental expression and subcellular localization. Titin is a giant sarcomeric protein responsible for the elasticity of striated muscle that may also function as a molecular scaffold for myofibrillar assembly. Molecular analysis and immunostaining with antibodies to multiple titin epitopes indicates that the chromosomal and muscle forms of titin may vary in their NH2 termini. The identification of titin as a chromosomal component provides a molecular basis for chromosome structure and elasticity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13