HPLC/DAD analysis of phenolic compounds from lavender and its application to quality control

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Areias, FM (Author) Other; Valentao, P (Author) Other; Andrade, PB (Author) FFUP; Moreira, MM (Author) Other; Amaral, J (Author) Other; Seabra, RM (Author) Other

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 23

Pages: 2563-2572

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 34 Citations

Scopus - 36 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-1SA

DOI: 10.1081/jlc-100100510

Abstract (EN): A reversed phase HPLC procedure is proposed for the determination of eight phenolic compounds (2-O-glucosilcoumaric acid, o-coumaric acid, rosmarinic acid, apigenin-7-O-glucoside, coumarin, herniarin, luteolin, and apigenin) in lavender. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS 2 (5 mu m particle size: 25.0 x 0.46 cm) column. From the several extractive solvents assayed, only ethanol was able to extract all the mentioned compounds. Best resolution was obtained using a gradient of water-formic acid (19:1) and acetonitrile. Ten samples were subjected to quantification, all of them showing a common composition pattern.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10