Rapid Fluorimetric Quantitation of Ibandronate by Coupling Quantum Dots and Multicommutated Flow Injection Analysis

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Ruiz Medina, A (Author) Other; Molina Garcia, L (Author) Other; Llorent Martinez, EJ (Author) Other; Santos, JLM (Author) FFUP; Fernandez de Cordova, ML (Author) Other

Journal

Title: Current Pharmaceutical Analysis

Vol. 9

Pages: 237-243

ISSN: 1573-4129

Publisher: Bentham Science

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 3 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-004-ZRA

DOI: 10.2174/1573412911309030002

Abstract (EN): Ibandronate is a potent nitrogen-containing bisphosphonate with a tertiary amine group, which does not easily form chromophore derivatives that can be detected by UV light of fluorescence emission. Here, a simple and straightforward automated multicommutated flow system, making use of water-soluble mercaptopropionic acid-capped CdTe quantum dots, was implemented for the fluorescence quantitation of ibandronate in pharmaceutical formulations. The developed approach was based on the analyte ability to establish surface interactions that resulted in quenched nanocrystals fluorescence intensity, being this effect proportional to the target compound's concentration. Size and concentration of quantum dots and pH of the media were some of the most relevant and influencing parameters studied. The proposed methodology allowed the determination of ibandronate in the range of 20-200 mu g mL(-1), with good repeatability (RSD<3%) and a sampling frequency of about 70 samples per hour. The results obtained in the analysis of pharmaceuticals showed excellent agreement with those provided by the manufacturer.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7