Enzyme based assays in a sequential injection format: A review

• Type: Another Publication in an International Scientific Journal
• Date: 2011

Authors

Cristina I C Silvestre (Author) Other; Paula C A G Pinto (Author) FFUP; Marcela A Segundo (Author) FFUP; Lucia L M F S Saraiva (Author) FFUP; Jose L F C Lima (Author) FFUP

Journal

Title: Analytica Chimica Acta

Vol. 689 No. 2

Pages: 160-177

ISSN: 0003-2670

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 36 Citations

Scopus - 41 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-002-T76

DOI: 10.1016/j.aca.2011.01.048

Abstract (EN): Sequential injection analysis systems have been extensively exploited in the last decades for the implementation of enzyme based assays aiming the evaluation of enzyme activity or the determination of specific analytes. The most prominent aspects of the automation of enzymatic assays in these systems are discussed in this review. Special attention is devoted to the mode of enzyme manipulation in homogeneous or heterogeneous media and to the comparison with batch and flow injection enzyme methodologies. The possibility of implementing strategies for the enhancement of selectivity in specific determinations is also addressed. The more recent trends in this field are discussed focusing mainly on the miniaturization resorting to the lab-on valve platform as well as on the bead injection concept.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 18