Voltammetric immunosensor for the diagnosis of celiac disease based on the quantification of anti-gliadin antibodies

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Marta M P S Neves (Author) Other; Maria B Begona Gonzalez Garcia (Author) Other; Alice Santos Silva (Author) FFUP; Agustin Costa Garcia (Author) Other

Journal

Title: Sensors and Actuators, B: Chemical

Vol. 163

Pages: 253-259

ISSN: 0925-4005

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 19 Citations

Scientific classification

FOS: Engineering and technology > Other engineering and technologies

Other information

Authenticus ID: P-002-CKH

DOI: 10.1016/j.snb.2012.01.048

Abstract (EN): Antibodies against gliadin are used to detect celiac disease (CD) in patients. An electrochemical immunosensor for the voltammetric detection of human anti-gliadin antibodies (AGA) IgA and AGA IgG in real serum samples is proposed. The transducer surface consists of screen-printed carbon electrodes modified with a carbon nanotube/gold nanoparticle hybrid system, which provides a very useful surface for the amplification of the immunological interactions. The immunosensing strategy is based on the immobilization of gliadin, the antigen for the autoantibodies of interest, onto the nanostructured surface. The antigen-antibody interaction is recorded using alkaline phosphatase labeled anti-human antibodies and a mixture of 3-indoxyl phosphate with silver ions (3-IP/Ag+) was used as the substrate. The analytical signal is based on the anodic redissolution of the enzymatically generated silver by cyclic voltammetry. The electrochemical behavior of this immunosensor was carefully evaluated assessing aspects as sensitivity, non-specific binding and matrix effects, and repeatability and reproducibility. The results were supported with a commercial ELISA test.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7