Development, validation, and application of an HPLC/UV method for quantification of casein in infant formulae and follow-up milks

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Ferreira, IMPLVO (Author) FFUP; Mendes, E (Author) Other; Marques, J (Author) Other; Ferreira, MA (Author) Other

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 23

Pages: 2057-2065

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 10 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-1S9

DOI: 10.1081/jlc-100100473

Abstract (EN): A simple, rapid and accurate HPLC method was used to determine the casein content in infant formulae and follow-up milks. The proposed methodology used a Chrompack 300 RP chromatographic column and an UV detector. Gradient elution was carried out with a mixture of two solvents. Solvent A was trifluoroacetic acid in water and solvent B was acetonitrile-water-trifluoroacetic acid. A linear relationship between the concentration of casein and the UV absorbance at 280 nm was obtained. This linearity was maintained over the concentration range 0.04-2.0 mg/mL. Preliminary studies made on processed milks showed that casein content in those products was not affected by heat treatment when compared with raw milk casein content. Hence, the quantification of casein in other milk products could be possible using this chromatographic column. The detection limit value was 0.02 mg/mL. The validity of the method was verified. For recovery studies several determinations were carried out. Four samples were analysed before and after the addition of known amounts of casein. Recovery values ranged between 80 and 98%. The precision of the method was also evaluated, and the %CV found was less than 2%. The developed methodology was applied to the monitorization of casein in twelve samples of infant formulae and follow-up milks. The mean values obtained were 7.5 +/- 2.8 (n=14) and 16.6 +/- 3.7 (n= 10), respectively.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9