Monosegmented flow analysis of slow enzymatic reactions: Determination of triglycerides in serum

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Araujo, AN (Author) FFUP; Catita, JAM (Author) Other; Lima, JLFC (Author) FFUP; Zagatto, EAG (Author) Other

Journal

Title: Fresenius' Journal of Analytical Chemistry

Vol. 360

Pages: 100-103

ISSN: 0937-0633

Publisher: Springer

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 9 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-94Z

DOI: 10.1007/s002160050650

Abstract (EN): A monosegmented flow system (MCFA) is proposed to achieve slow enzymatic spectrophotometric determinations, here applied to the determination of triglycerides in blood serum. The sample (4.5 mu L), enzymatic reagent (150 mu L) and an air plug (100 mu L) are simultaneously inserted into a carrier stream buffered to pH 7.9 (Tris.HCl). In order to avoid the cumbersome step of air removal, a relocating detector was used. This system handles about 60 samples/h, yielding precise results (r.s.d. usually < 2.5%). Sensitivity is 56 mAU.L/mmol up to 6 mmol/L triglycerides. Accuracy was assessed by running 50 samples already analysed by a conventional procedure yielding the equation C-MCFA(mmol/L) = 1.00(+/- 0.04) C-Ref(mmol/L)- 0.03(+/- 0.08); r = 0.990.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4