Fosfomycin increases chromosome instability in lymphocytes from Fanconi Anemia patients

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Rosa Sousa (Author) Other; Filipa Ponte (Author) Other; Sara Teixeira (Author) Other; Laia A Andrade (Author) Other; Cristina Goncalves (Author) Other; Jose Barbot (Author) Other; Jorge Coutinho (Author) Other; Felix Carvalho (Author) FFUP; Beatriz Porto (Author) ICBAS

Journal

Title: Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Vol. 754

Pages: 58-62

ISSN: 1383-5718

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 1 Citation

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-006-A3R

DOI: 10.1016/j.mrgentox.2013.04.005

Abstract (EN): Fanconi Anemia (FA) is a chromosome instability (Cl) syndrome, clinically characterized by progressive bone marrow failure and increased cancer predisposition. Lymphocytes from FA patients have hypersensitivity to alkylating agents, particularly to diepoxybutane (DEB). The antibiotic fosfomycin (FOM) is an alkylating agent. FOM is used as a large spectrum antibiotic and also as a prophylactic pre-surgery agent. FOM has been considered non-genotoxic. However, no specific genotoxic evaluation directed to patients with hypersensitivity to alkylating agents was performed. As FA patients are very susceptible to infections and may be submitted to several surgeries. FOM can eventually be prescribed to them during their lifetime. In the present study we evaluated the putative genotoxic effect of FOM in cultured lymphocytes from FA patients, compared to cultured lymphocytes from healthy donors (HD). Cultures from FA patients and HD were treated with 0.5 mM FOM or with 0.6 mM DEB and Cl was evaluated. Results showed that FOM significantly increases Cl in cultured lymphocytes from FA patients, compared to lymphocytes from HD, in which no effect was found. Additionally, a direct correlation between DEB and FOM toxicity was observed in lymphocytes from FA patients, indicating similar susceptibility to both agents.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5