Code: | EBE0188 | Acronym: | ENGP |
Keywords | |
---|---|
Classification | Keyword |
OFICIAL | Biotechnology |
Active? | Yes |
Responsible unit: | Department of Chemical Engineering |
Course/CS Responsible: | Master in Bioengineering |
Acronym | No. of Students | Study Plan | Curricular Years | Credits UCN | Credits ECTS | Contact hours | Total Time |
---|---|---|---|---|---|---|---|
MIB | 13 | Syllabus | 3 | - | 6 | 56 | 162 |
MIEQ | 12 | Syllabus | 4 | - | 6 | 56 | 162 |
Students should learn basic concepts related to proteins, their application, relationships between composition, structure and function, as well as characterization methods.
Students should also get familiar with concepts related to proteins activity, stability, kinetics and synthesis in physiological conditions and industrial application of recombinant DNA technology, by using several strategies of protein engineering.
Technical skills:
Understanding basic concepts related to proteins, their application, relationships between composition, structure and function, as well as characterization methods.
Students should also get familiar with concepts related to proteins activity, stability, kinetics and synthesis in physiological conditions and industrial application of recombinant DNA technology, by using several strategies of protein engineering.
Non-technical skills:
Group work and oral presentation.
Program of theoretical-practical classes
1- Proteins in medicine and industry
2- Proteins- composition, structure and function
3- Proteins- detection and characterization
4- Enzymes- Kinetics, activity, stability and regulation
5- Main objectives of protein engineering
6- Protein synthesis- Transcription
7- Protein synthesis- Post-transcriptional events
8- Protein synthesis- Translation
9- Protein synthesis- Post-translational events
10- Recombinant proteins expression- advantages and hosts
11- Recombinant proteins expression- methods for molecular cloning
12- Protein Engineering- Vector design
13- Protein Engineering- Rational design of proteins
14- Protein Engineering- Random strategies
15- Protein Engineering- Hybrid strategies
Program of laboratory classes
a) Production, purification and analysis of a recombinant protein
Classes will be based on the oral presentation of the themes of the course unit with the support of the board and slides. Students will be asked to participate in classes and to use a deductive reasoning.
Laboratory classes will be based on practical assignments, which illustrate the contents of the course unit.
Designation | Weight (%) |
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Exame | 60,00 |
Prova oral | 20,00 |
Trabalho laboratorial | 20,00 |
Total: | 100,00 |
Participation in the laboratory sessions and delivery of the final report with a positive (greater or equal to 10/20) classification. Delivery of the group work (along with respective oral presentation) with positive classification. Participation on the TP classes according to the evaluation guidelines from. FEUP. For the lab sessions, students must attend more than 75% of the scheduled classes. The final lab grade will be multiplied by the percentage of lab sessions that the student has effectively attended (from beginning to end).
Final Grade (FG) will be based on the following formula:
FG= 0.6xE + 0.2xL + 0.2xT
E- Final Exam
L- Final report of laboratory assignments
T - Grou pwork classification (includes quality of oral presentation)
For the TP classes, one group work will have to be delivered and presented orally to the class. The subject of this work will be decided in the first classes of the semester. For the laboratory part, a final report will also have to be delivered along with a short presentation of the lab session.
In case there is a determination by the authorised bodies from U. Porto that lab classes are not possible, the weights of the different components may be re-adjusted.
According to the rules students have to attend an exam at recurso (resit) season.
Students can complete the laboratory component, by doing one experimental session (to be schedulled according to the lab convenience), writing a full report of the experiental work, and give an orla presentation about their work an results . Students must also present the TP work (which will be done individually) in printed form as well as in an oral presentation.
Students can improve their grades by attending recurso (resit) exam. Students cannot improve the grade of the laboratory component nor the grade obtained on the TP group work.