Separation Processes in Biotecnology

Code:

EBE0133

Acronym:

PSBI

Keywords
Classification	Keyword
OFICIAL	Biological Engineering

Instance: 2019/2020 - 1S

Active?	Yes
Responsible unit:	Department of Chemical Engineering
Course/CS Responsible:	Master in Bioengineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIB	26	Syllabus	4	-	6	56	162
MIEQ	8	Syllabus	4	-	6	56	162
			5

Teaching language

Portuguese

Objectives

To acquire advanced engineering knowledge in purification processes of biological products, as well as their approval and regulation. To develop students’ reasoning skills, as well as their capacity to analyse production diagrams and to use bioinformatic tools.

Learning outcomes and competences

Learn how to analyze an industrial production process particularly the operation units concerning downstream processing. Learn how to select the most adequate equipment for each operation unit and to perform equipment sizing in particular cases.

Working method

Presencial

Program

1- Biotechnology and bioproducts 2- Introduction to the purification of biological products 3- Cell rupture a) Mechanical methods b) Non-mechanical methods 4- Solid-liquid separation a) Sedimentation b) Centrifugation c) Conventional filtration d) Membrane processes 5- Chromatography a) Gel filtration b) Ionic exchange c) Affinity d) Hydrophobic interaction 6- Extraction processes 7- Precipitation and crystallization 8- Solvent removal a) Drying b) Freeze drying c) Evaporation 9- Product activity 10- Regulation a) Validation b) Approval c) Formulation 11-Bioinformatic analysis of proteins 12- Analysis of production diagrams 13- Simulation of purification processes

Mandatory literature

Harrison, Roger G. 070; Bioseparations science and engineering. ISBN: 0-19-512340-9
Filipe Mergulhão; Textos de apoio da disciplina, 2009. ISBN: (A fornecer aos alunos em formato electrónico)

Complementary Bibliography

Crommelin, Daan J. A. 340; Pharmaceutical biotechnology. ISBN: 0-415-28501-1
Wink, M.; An introduction to molecular biotechnology, Wiley-VCH, 2006. ISBN: 3-527-31412-1

Teaching methods and learning activities

Oral presentation with the support of the board and slides. Classes at the computer room using simulation software and bioinformatic tools available on the web.

keywords

Natural sciences > Biological sciences > Biological engineering
Technological sciences > Engineering

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Designation	Weight (%)
Trabalho escrito	100,00
Total:	100,00

Eligibility for exams

According to General Evaluation Rules of FEUP, students have to attend classes.

Calculation formula of final grade

Final grade will be obtained by an weighted average of the several components of the final work, the classification from the oral presentations and the log book. The respective weights wil be discussed on class.

Examinations or Special Assignments

Students will have to do a group assignment, being the assignment sheet delivered at the beginning of the semester. The assignment is to be carried out during the semester (some parts will be carried out in class). The group assignment comprises: a) Introduction b) Patent research c) Development of a production diagram d) Bioinformatic analysis of proteins e) Simulation of purification processes f) Conclusion. This work will be orally presented and discussed in class.

Special assessment (TE, DA, ...)

By globla exam on the resit period. Admittance to that exam requires the delivery of the group work with all its components and with a final evaluation of at least 7/20 points.

Classification improvement

Students can improve their grades by attending an exam at the recurso (resit) season.