Bioengineering A-II

Code:

EQ419

Acronym:

BIOAII

Instance: 2005/2006 - 2S

Active?	Yes
Responsible unit:	Department of Chemical and Biological Engineering
Course/CS Responsible:	Chemical Engineering

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
LEQ	13	Plano Oficial EQ a partir 2003	4	3,5	5,5	-
		Plano para Bachareis EQ a partir de 1996	1	3,5	5,5	-

Teaching language

Portuguese

Objectives

After the acquisition of basic concepts of general microbiology (Bioengenharia AI), the student is introduced to diverse biotechnological applications of microorganisms.

Program

THEORETICAL COMPONENT:
1. INTRODUCTION TO BIOTECHNOLOGY - History and sectors of activity.
2. ORGANIZATION OF BIOTECHNOLOGICAL PROCESSES
2.1 Substrates
2.2 Sterilisation methods
2.3 Organisms
2.4 Strain development
2.5 Preservation of organisms
2.6 Methods of fermentation
2.7 Product recovery
3. THE IMPACT OF THE MICROORGANISMS ON DIFFERENT ACTIVITY SECTORES. EXEMPLES OF BIOTECHNOLOGICAL PROCESSES
3.1 Food industry
3.1.1 Beneficial / pernicious microorganisms
3.1.2 Methods of food preservation
3.1.2.1 Cheese production
3.1.2.1 Wine production
3.1.3 Bacterial quality control
3.2 Chemical industry
3.2.1 Ethanol production
3.2.2 Glycerol production
3.2.3 Citric acid production
3.3 Pharmaceutical industry
3.3.1 Penicillin production
3.4 Microbial leaching
3.5 Bioremediation
3.5.1 Waste water treatment
3.6 Prevention of environmental contamination
3.6.1 Bio-insecticides
3.6.2 Biodegradable polymers
LABORATORY WORK:
1. Yoghurt production. Survival of potential pathogens in yoghurt.
2. Lactic acid production by Lactobacillus pentosus or yoghurt.
Yield of lactic acid fermentation.
3. Bacteriological examination of potable water

The students will visit a pharmaceutical industry or a food industry and a waste water treatment plant.

Mandatory literature

Crueger, Wulf; Biotechnology, N. ISBN: 0-87893-135-X

Complementary Bibliography

Stryer, Lubert; Biochemistry, N. ISBN: 0-7167-2009-4
Glick, Bernard R.; Molecular biotechnology, N. ISBN: 1-55581-269-4
Battistotti, Bruno 070; Quesos del mundo, N. ISBN: 84-85791-41-X
Glazer, Alexander N.; Microbial biotechnology, N. ISBN: 0-7167-2608-4
Bitton, Gabriel; Wastewater Microbiology, N. ISBN: 0-471-30986-9
Hurst, Christon J. 340; Manual of environmental microbiology, N. ISBN: 1-55581-199-X
Demain, Arnold L. 340; Manual of industrial microbiology and biotechnology, N. ISBN: 1-55581-128-0
Pelczar, Jr., Michael Joseph; Microbiologia, N. ISBN: 85-346-0196-8 (vol.1)
Madigan, Michael T; Brock biology of microorganisms, N. ISBN: 0-13-049147-0

Teaching methods and learning activities

The methodology followed can be described as follow:

LECTURES:
Oral exposition of the subjects supported by the blackboard and transparencies. Students are motivated to participate and to deduce the subjects.

LABORATORY WORK:
Experimental works are performed. Notions of safety and laboratory good practices are emphasised. Methodologies used and interpretation of experimental results are discussed with the students.

Software

Excel, Power Point e Word

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Description	Type	Time (hours)	Weight (%)	End date
Subject Classes	Participação presencial	52,00
Report	Trabalho escrito			2006-06-01
Short-test	Exame			2006-06-01
Seminar	Trabalho escrito			2006-05-25
	Total:	-	0,00

Amount of time allocated to each course unit

Description	Type	Time (hours)	End date
Avaliação contínua	Frequência das aulas
	Total:	0,00

Eligibility for exams

- The laboratory works are compulsory for all the students, including those under the article 4th, paragraph a) and b).
- Students approved at the laboratory work of the discipline since the scholar year 2001/2002, do not need to repeat the lab works.
- The minimal grade at each of the evaluation components is 10.
- To be approved the student must have final grades ≥ 10 values in both laboratory work and each of the theoretical components.

Calculation formula of final grade

Laboratory work:
GLW= (0.3 x CE) +(0.7 x REP)

Final Grade:
GF = (0.3 x GLW) + (0.4 x ST) + (0.3 x SEM)
where,
ST – Short test;
SEM – Seminar;
CE – Continuous evaluation (during the lab classes);
REP – Report of laboratory experimental works;

Special assessment (TE, DA, ...)

- Students approved at the laboratory work of the subject since the scholar year 2001/2002 will be evaluated only at the theoretical component of the subject in a written exam.

Classification improvement

- Students that pretend to improve their final grade, can only improve the theoretical grade in a written exam.

Observations

- Each group of two students must present a report of an experimental work performed during the semester (REP). The report should be divided into the following sections: Title, summary, introduction, materials and methods, results and discussion, conclusions and bibliography.
- The theoretical component of the subject will be evaluated in two different periods by a short test and a seminar. The short test is a written test without bibliographic support about theoretical subjects and experimental works, and should be performed within 1,5 h. The seminar is an oral exposition of a free theme of applied microbiology, presenting the organisms involved in the process and theprocess itself.