Automatic Information Extraction from Gel Electrophoresis Images Using GEIAS

• Type: Article in International Conference Proceedings Book
• Date: 2010

Authors

Caridade, CMR (Author) Other; Marcal, ARS (Author) FCUP; Mendonca, T (Author) FCUP; Pessoa, AM (Author) Other; Pereira, S (Author) FCUP

Conference proceedings International

Title: Image Analysis and Recognition - Part II

Pages: 185-194

7th International Conference, ICIAR 2010

Póvoa de Varzim, Portugal, June 21-23, 2010

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Computer and information sciences

Other information

Authenticus ID: P-003-B7H

DOI: 10.1007/978-3-642-13775-4_19

Abstract (EN): This paper presents a method (GEIAS) for the automatic processing of digital images obtained from Gel Electrophoresis. The performance of GEMS was tested using 12 images, obtained from 4 gels with 3 different exposures with a total of 1082 bands, comparing the results provided by GEIAS and 3 other software tools. The GEIAS is able to fully automatically detect; DNA lanes while the other 3 software tools tested can only do this in a semi-automatic or manual way. For the correct location of DNA bands, GEIAS required a manual correction of the location in 10.0% of the bands, and the other software tools 13.0%, 15.0% and 25.4%. The average error in the estimation of molecular weight was tested using a total of 5443 bands in 12 image using 672 reference/observed lane pairs. The average error was found to be 9.2% for GEIAS and 11.2%, 14.4% and 13.1% for the other software tools tested.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10