Volatile composition of Catharanthus roseus (L.) G. Don using solid-phase microextraction and gas chromatography/mass spectrometry

• Type: Article in International Scientific Journal
• Date: 2009

Authors

De Pinho, PG (Author) Other; Rui F Goncalves (Author) Other; Patricia Valentao (Author) FFUP; David M Pereira (Author) Other; Rosa M Seabra (Author) Other; Paula B Andrade (Author) FFUP; Mariana Sottomayor (Author) FCUP

Journal

Title: Journal of Pharmaceutical and Biomedical Analysis

Vol. 49 No. 3

Pages: 674-685

ISSN: 0731-7085

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 45 Citations

ISI Web of Science

Scopus - 51 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-KVW

DOI: 10.1016/j.jpba.2008.12.032

Resumo (PT): A total of 88 volatile and semi-volatile components were formally or tentatively identified in flowers, leaves and stems of Catharanthus roseus (L.) G. Don (cv. Little Bright Eye), by headspace solid-phase microextraction (HS-SPME) and by dichloromethane extraction, combined with gas chromatography–mass spectrometry (GC–MS). These include some diterpenic compounds (manool and manoyl oxides), a sesquiterpen (α-bisabolol), and some pyridine, pyrazine, indol and carotenoid derivatives. Applying multivariate analysis (principal component analysis and agglomerative hierarchic cluster analysis) to the HS-SPME–GC–MS data, it was possible to characterize each part of the vegetal material using a relative small number of compounds. Hence, flowers were richer in terpenic molecules (including limonene), α-bisabolol, methyljasmonate, cis-jasmone, 2-phenylethanol, phenylacetaldehyde, trans-2-octenal, benzylic alcohol and 2-isobutyl-3-methoxypyrazine. Leaves can be characterized by the methyl and propyl esters of fatty acids, mono- and disaturated, trans-phytol, carotenoid derivative compounds, hydrofarnesylacetone, methylanthranilate, manool and epi-manool oxide, while stems have high levels of volatile aldehydes, such as hexanal, octanal, cis-2-nonenal, cis-2-decenal, cis, trans-2,6-nonadienal, trans, trans-2,4-decadienal and cis, trans-2,4-decadienal. Dichloromethane extraction allowed also the identification of some alkaloid-like compounds that were not detected by HS-SPME. <br> <br> Keywords: Catharanthus roseus (L.) G. Don; Volatiles and semi-volatiles; HS-SPME; GC–MS <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGX-4V8GB48-2&_user=2460038&_coverDate=04%2F05%2F2009&_rdoc=16&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235266%232009%23999509996%23961074%23FLA%23display%23Volume)&_cdi=5266&_sort=d&_docanchor=&_ct=47&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=99276bfa397e5841cc7a20898c62f8c9"> Texto integral</a> <br> <br>

Abstract (EN): A total of 88 volatile and semi-volatile components were formally or tentatively identified in flowers, leaves and stems of Catharanthus roseus (L) G. Don (cv. Little Bright Eye), by headspace solid-phase microextraction (HS-SPME) and by dichloromethane extraction, combined with gas chromatography-mass spectrometry (GC-MS). These include some diterpenic compounds (manool and manoyl oxides), a sesquiterpen (alpha-bisabolol), and some pyridine, pyrazine, indol and carotenoid derivatives. Applying multivariate analysis (principal component analysis and agglomerative hierarchic cluster analysis) to the HS-SPME-GC-MS data, it was possible to characterize each part of the vegetal material using a relative small number of compounds. Hence, flowers were richer in terpenic molecules (including limonene), alpha-bisabolol, methyljasmonate, cis-jasmone, 2-phenylethanol, phenylacetaldehyde, trans-2-octenal, benzylic alcohol and 2-isobutyl-3-methoxypyrazine. Leaves can be characterized by the methyl and propyl esters of fatty acids, mono- and disaturated, trans-phytol, carotenoid derivative compounds, hydrofarnesylacetone, methylanthranilate, manool and epi-manool oxide, while stems have high levels of volatile aldehydes, such as hexanal, octanal, cis-2-nonenal, cis-2-decenal, cis, trans-2,6-nonadienal, trans, trans-2,4-decadienal and cis, trans-2,4-decadienal. Dichloromethane extraction allowed also the identification of some alkaloid-like compounds that were not detected by HS-SPME.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12