Hydrocinnamic Acid and Perillyl Alcohol Potentiate the Action of Antibiotics against Escherichia coli

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Sousa, M (Author) Other; Afonso, AC (Author) Other; Teixeira, LS (Author) Other; Anabela Borges (Author) FEUP; Saavedra, MJ (Author) Other; Simões, LC (Author) Other; Simões, M (Author) Other; Simões M (Author) FEUP

Journal

Title: Antibiotics

Vol. 12

Final page: 360

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

ISI Web of Science

Scopus - 0 Citations

Associated Projects

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

GermIrrad - Sistema automatizado de irradiação germicida

Other information

Authenticus ID: P-00X-WA1

DOI: 10.3390/antibiotics12020360

Abstract (EN): The treatment of bacterial infections has been troubled by the increased resistance to antibiotics, instigating the search for new antimicrobial therapies. Phytochemicals have demonstrated broad-spectrum and effective antibacterial effects as well as antibiotic resistance-modifying activity. In this study, perillyl alcohol and hydrocinnamic acid were characterized for their antimicrobial action against Escherichia coli. Furthermore, dual and triple combinations of these molecules with the antibiotics chloramphenicol and amoxicillin were investigated for the first time. Perillyl alcohol had a minimum inhibitory concentration (MIC) of 256 mu g/mL and a minimum bactericidal concentration (MBC) of 512 mu g/mL. Hydrocinnamic acid had a MIC of 2048 mu g/mL and an MBC > 2048 mu g/mL. Checkerboard and time-kill assays demonstrated synergism or additive effects for the dual combinations chloramphenicol/perillyl alcohol, chloramphenicol/hydrocinnamic acid, and amoxicillin/hydrocinnamic acid at low concentrations of both molecules. Combenefit analysis showed synergism for various concentrations of amoxicillin with each phytochemical. Combinations of chloramphenicol with perillyl alcohol and hydrocinnamic acid revealed synergism mainly at low concentrations of antibiotics (up to 2 mu g/mL of chloramphenicol with perillyl alcohol; 0.5 mu g/mL of chloramphenicol with hydrocinnamic acid). The results highlight the potential of combinatorial therapies for microbial growth control, where phytochemicals can play an important role as potentiators or resistance-modifying agents.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 23