The effects of emerging contaminants on the behaviour of Acinetobacter calcoaceticus derived from biofilms

• Type: Article in International Scientific Journal
• Date: 2022-11-14

Authors

Inês Gomes (Author) FEUP; Simões M (Author) FEUP; Isabel C. Pinto (Author) FEUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY

ISSN: 2053-1400

Indexing

ISI Web of Knowledge

ISI Web of Science

Scopus

Associated Projects

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

Biocide_for_Biofilm - Desenvolvimento de novas soluções biocidas para o controlo efetivo de biofilmes

GermIrrad - Sistema automatizado de irradiação germicida

Healthy Waters - Healthy Waters - Identification, Elimination, Social Awareness and Education of Water Chemical and Biological Micropollutants with Health and Environmental Implications

Other information

DOI: 10.1039/D2EW00246A

Abstract (EN): Knowledge regarding the ecotoxicological effects of emerging contaminants (EC) in drinking water distribution system (DWDS) microbiota is limited. Given the issues associated with biofilm formation in DWDS, this work studied the effects of the exposure of Acinetobacter calcoaceticus biofilms to four pharmaceutical compounds already encountered in drinking water (DW) at residual concentrations (ng L−1): caffeine – CAF, carbamazepine – CBZ, ciprofloxacin – CIP and ibuprofen – IBP. The exposure assays were performed with the compounds alone and in combination (MIX). The results obtained aim to contribute for the understanding of the role of ECs exposure for 7 days in bacterial behaviour, particularly the ability to form biofilms, susceptibility to antibiotics (CIP, levofloxacin – LEV, tetracycline – TET and trimethoprim–sulfamethoxazole – TMP-SMX), tolerance to NaOCl, motility and growth rate. It was found that IBP exposure decreased biofilm formation ability by 11%, while MIX exposure increased it by 16%. Regarding antibiotics susceptibility, CAF exposure decreased susceptibility to TPM-SMX, while CBZ and CIP exposures seem to cause an increase in the susceptibility to TMP-SMX. Furthermore, exposure to residual levels of CIP decreased the susceptibility to CIP and LEV, and IBP exposure seems to slightly decrease the susceptibility to LEV and TET. The ranges of minimum bactericidal concentration (MBC) of NaOCl have also slightly increased (for CIP and IBP exposure) or decreased (for the exposure to CBZ). Higher A. calcoaceticus growth rates were obtained after CIP exposure. These results demonstrate that the selected ECs affect the behaviour and growth dynamics of bacteria under conditions typically found in DWDS. Among the selected ECs, CIP and IBP were those causing the most significant effects. Furthermore, this study lays the groundwork for knowledge enrichment regarding bacterial behaviour in biofilms and their impact on DWDS.

Language: English

Type (Professor's evaluation): Scientific