Resveratrol induces ordered domains formation in biomembranes: Implication for its pleiotropic action

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Neves, AR (Author) Other; Nunes, C (Author) Other; Salette Reis (Author) FFUP

Journal

Title: Biochimica et Biophysica Acta: Biomembranes

Vol. 1858

Pages: 12-18

ISSN: 0005-2736

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 21 Citations

Other information

Authenticus ID: P-00J-YV5

DOI: 10.1016/j.bbamem.2015.10.005

Abstract (EN): Resveratrol is a polyphenol compound with great value in cancer therapy, cardiovascular protection, and neuro-degenerative disorders. The mechanism by which resveratrol exerts such pleiotropic effects is not yet clear and there is a huge need to understand the influence of this compound on the regulation of lipid domains formation on membrane structure. The aim of the present study was to reveal potential molecular interactions between resveratrol and lipid rafts found in cell membranes by means of Forster resonance energy transfer, DPH fluorescence quenching, and triton X-100 detergent resistance assay. Liposomes composed of egg phosphatidylcholine, cholesterol, and sphingomyelin were used as model membranes. The results revealed that resveratrol induces phase separation and formation of liquid-ordered domains in bilayer structures. The formation of such tightly packed lipid rafts is important for different signal transduction pathways, through the regulation of membrane-associating proteins, that can justify several pharmacological activities of this compound.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7