Crosstalk between Helicobacter pylori and Gastric Epithelial Cells Is Impaired by Docosahexaenoic Acid

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Correia, M (Author) Other; Michel, V (Author) Other; Osorio, H (Author) FMUP; El Ghachi, M (Author) Other; Bonis, M (Author) Other; Boneca, IG (Author) Other; De Reuse, H (Author) Other; Matos, AA (Author) Other; Lenormand, P (Author) Other; Seruca, R (Author) FMUP; Figueiredo C (Author) FMUP; Machado JC (Author) FMUP; Touati, E (Author) Other

Journal

Title: PLoS ONE

Vol. 8 No. 1

Final page: e60657

ISSN: 1932-6203

Publisher: Public Library of Science

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 18 Citations

Other information

Authenticus ID: P-005-1N6

DOI: 10.1371/journal.pone.0060657

Abstract (EN): H. pylori colonizes half of the world's population leading to gastritis, ulcers and gastric cancer. H. pylori strains resistant to antibiotics are increasing which raises the need for alternative therapeutic approaches. Docosahexaenoic acid (DHA) has been shown to decrease H. pylori growth and its associated-inflammation through mechanisms poorly characterized. We aimed to explore DHA action on H. pylori-mediated inflammation and adhesion to gastric epithelial cells (AGS) and also to identify bacterial structures affected by DHA. H. pylori growth and metabolism was assessed in liquid cultures. Bacterial adhesion to AGS cells was visualized by transmission electron microscopy and quantified by an Enzyme Linked Immunosorbent Assay. Inflammatory proteins were assessed by immunoblotting in infected AGS cells, previously treated with DHA. Bacterial total and outer membrane protein composition was analyzed by 2-dimensional gel electrophoresis. Concentrations of 100 mu M of DHA decreased H. pylori growth, whereas concentrations higher than 250 mu M irreversibly inhibited bacteria survival. DHA reduced ATP production and adhesion to AGS cells. AGS cells infected with DHA pre-treated H. pylori showed a 3-fold reduction in Interleukin-8 (IL-8) production and a decrease of COX2 and iNOS. 2D electrophoresis analysis revealed that DHA changed the expression of H. pylori outer membrane proteins associated with stress response and metabolism and modified bacterial lipopolysaccharide phenotype. As conclusions our results show that DHA anti-H. pylori effects are associated with changes of bacteria morphology and metabolism, and with alteration of outer membrane proteins composition, that ultimately reduce the adhesion of bacteria and the burden of H. pylori-related inflammation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9