Project: PTDC/BTA-BTA/2249/2021

Project name:	CM4Methane - Unravelling the role of conductive materials in the acceleration of methane production from waste in anaerobic digestion processes
Project code:	PTDC/BTA-BTA/2249/2021
Main Objective:	Reforçar a investigação, o desenvolvimento tecnológico e a inovação
Intervention Region:	Norte
Proposing institution/Lead promoter/Coordinating entity:	Universidade do Minho
Partner(s)/Co-promoter(s)/Participating institution(s):	Faculdade de Engenharia da Universidade do Porto
Date of approval:	2021-10-15
Start date:	2022-01-01
Completion date:	2025-06-30

Eligible Cost of the Project

Total Eligible Cost:	249.838,88 EUR
Eligible Cost in the University of Porto:	76.518,80 EUR
Faculdade de Engenharia da Universidade do Porto:	76.518,80 EUR

Total Financial Support

Orçamento de Estado:

249.838,88 EUR

Financial Support to the University of Porto

Total of the University of Porto:	76.518,80 EUR
Nacional/Regional \| Orçamento de Estado \| Faculdade de Engenharia da Universidade do Porto:	76.518,80 EUR

Objectives, activities and expected/achieved results

The main objective of this project is to elucidate the role of conducting materials (CM) in the transfer of electrons in anaerobic biological processes, studying the mechanisms of electron transfer in methanogenic consortia in the presence of CM. With a multitasking approach and in sequential steps we will 1) synthesize materials with acidic functional groups (eg, carboxyl, phenol, hydroxyl) and basic groups (eg, amine, methyl), necessary to find the most important factor/property in improving the production of CH4; 2) carry out batch incubations with pure methanogenic cultures and synthetic syntrophic cocultures, in the presence of commercial and functionalized CM, but also expanded (non-conductive) glass, to clarify the effects of these materials on the activity of different trophic groups, as well as about syntrophic interactions; 3) study the feasibility of treating real WWTP sludge (samples from SIMDOURO and/or Águas do Norte) operating a sequential anaerobic reactor with and without CM, to quantify the impact of CM in the production of biogas; and 4) evaluate the physiological and metabolic changes in pure cultures of methanogenic microorganisms, syntrophic cocultures and complex communities incubated with CM by (meta)genomic and (meta)transcriptomic analyses.

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