Evaluation of the immune response following a short oral vaccination schedule with hepatitis B antigen encapsulated into alginate-coated chitosan nanoparticles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Olga Borges (Autor) Outra; Joana Tavares (Autor) FFUP; Adriano de Sousa (Autor) Outra; Gerrit Borchard (Autor) Outra; Hans E. Junginger (Autor) Outra; Anabela Cordeiro-da-Silva (Autor) FFUP

Revista

Título: European Journal of Pharmaceutical Sciences

Vol. 32 Nº 4

Páginas: 278-290

ISSN: 0928-0987

Editora: Elsevier

Indexação

ISI Web of Knowledge - 77 Citações

Scopus - 104 Citações

Pubmed / Medline

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-004-5YR

Resumo (PT): The purpose of this work was to assess the ability of recombinant hepatitis B vaccine, encapsulated in alginate-coated chitosan nanoparticles, to induce local and systemic immune responses following oral vaccination. The antigen was administered either alone or in combination with the immunopotentiator, synthetic oligodeoxynucleotide containing immunostimulatory CpG motif (CpG ODN) as adjuvant, and associated or not with the alginate-coated chitosan nanoparticles. After two immunizations the group I (HBsAg associated with nanoparticles) and the group VI (HBsAg and CpG, both associated with nanoparticles) showed enhancedimmune responses. Both groups showed significant higher values of the CD69 expression in CD4+ and CD8+ T-lymphocytes and lower values of this marker in B lymphocytes. Moreover, a strongest proliferative response of the splenocytes, ex vivo stimulated with concanavalin A, was observed in the same groups. Although with a presence of non-responder mice within the groups, only mice of the groups I and VI elicited the generation of anti-HBsAg antibodies detected in serum (IgG) and in the intestinal washings (sIgA). The results demonstrated that coated chitosan nanoparticles might have potential for being used as a deliver system for oral vaccination with the recombinant hepatitis B surface antigen. <br> <br> Keywords: Oral vaccination Hepatitis B surface antigen CpG oligodeoxynucleotide Alginate-coated chitosan nanoparticles Vaccines <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T25-4PF1WCM-2&_user=2460038&_coverDate=12%2F31%2F2007&_rdoc=6&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234909%232007%23999679995%23673524%23FLA%23display%23Volume)&_cdi=4909&_sort=d&_docanchor=&_ct=18&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=c1242ce5a2c96dbca4ec6e2b2be43575">Texto integral</a> <br> <br>

Abstract (EN): The purpose of this work was to assess the ability of recombinant hepatitis B vaccine, encapsulated in alginate-coated chitosan nanoparticles, to induce local and systemic immune responses following oral vaccination. The antigen was administered either alone or in combination with the immunopotentiator, synthetic oligodeoxynucleotide containing immunostimulatory CpG motif (CpG ODN) as adjuvant, and associated or not with the alginate-coated chitosan nanoparticles. After two immunizations the group I (HBsAg associated with nanoparticles) and the group VI (HBsAg and CpG, both associated with nanoparticles) showed enhancedimmune responses. Both groups showed significant higher values of the CD69 expression in CD4+ and CD8+ T-lymphocytes and lower values of this marker in B lymphocytes. Moreover, a strongest proliferative response of the splenocytes, ex vivo stimulated with concanavalin A, was observed in the same groups. Although with a presence of non-responder mice within the groups, only mice of the groups I and VI elicited the generation of anti-HBsAg antibodies detected in serum (IgG) and in the intestinal washings (sIgA). The results demonstrated that coated chitosan nanoparticles might have potential for being used as a deliver system for oral vaccination with the recombinant hepatitis B surface antigen. <br> <br> Keywords: Oral vaccination Hepatitis B surface antigen CpG oligodeoxynucleotide Alginate-coated chitosan nanoparticles Vaccines <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T25-4PF1WCM-2&_user=2460038&_coverDate=12%2F31%2F2007&_rdoc=6&_fmt=high&_orig=browse&_srch=doc-info(%23toc%234909%232007%23999679995%23673524%23FLA%23display%23Volume)&_cdi=4909&_sort=d&_docanchor=&_ct=18&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=c1242ce5a2c96dbca4ec6e2b2be43575"> Full text</a> <br> <br>

Idioma: Português

Tipo (Avaliação Docente): Científica