An improved sampling approach in multi-pumping flow systems applied to the spectrophotometric determination of glucose and fructose in syrups

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Carneiro, JMT (Autor) Outra; Dias, ACB (Autor) Outra; Zagatto, EAG (Autor) Outra; Santos, JLM (Autor) FFUP; Lima, JLFC (Autor) FFUP

Revista

Título: Analytica Chimica Acta

Vol. 531 Nº 2

Páginas: 279-284

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 21 Citações

ISI Web of Science

Scopus - 25 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-000-4P3

DOI: 10.1016/j.aca.2004.10.038

Resumo (PT): A novel sampling approach in flow analysis exploiting the multi-pumping concept is proposed and applied to the spectrophotometric determination of glucose and fructose in invert sugar syrups. The samples are collected in gelatin capsules and brought to the laboratory. The capsules are placed directly into the dissolution chamber of a flow-batch system, therefore, the manual sample dissolution step is not required. All the involved analytical steps, including the capsule dissolution under acidic conditions, are efficiently controlled by using discretely actuated solenoid micro-pumps. Glucose and fructose determinations involve sugar oxidation by periodate and evaluation of the remaining periodate by monitoring the tri-iodide complex formed after addition of potassium iodide. The proposed system is very stable, handles about 50 samples per hour and requires 28.8 μg NaIO4 and 2.5 mg KI per determination. Precise results (R.S.D. < 2%) in agreement with HPLC are obtained. <br> <br> Keywords: Sampling; Flow analysis; Spectrophotometry; Multi-pumping; Syrup analysis <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TF4-4F7H56H-2&_user=2460038&_coverDate=02%2F28%2F2005&_rdoc=16&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235216%232005%23994689997%23571620%23FLA%23display%23Volume)&_cdi=5216&_sort=d&_docanchor=&_ct=21&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=e50fca16813a85d57626bcab80a233ad"> Texto integral</a> <br> <br>

Abstract (EN): A novel sampling approach in flow analysis exploiting the multi-pumping concept is proposed and applied to the spectrophotometric determination of glucose and fructose in invert sugar syrups. The samples are collected in gelatin capsules and brought to the laboratory. The capsules are placed directly into the dissolution chamber of a flow-batch system, therefore, the manual sample dissolution step is not required. All the involved analytical steps, including the capsule dissolution under acidic conditions, are efficiently controlled by using discretely actuated solenoid micro-pumps. Glucose and fructose determinations involve sugar oxidation by periodate and evaluation of the remaining periodate by monitoring the tri-iodide complex formed after addition of potassium iodide. The proposed system is very stable, handles about 50 samples per hour and requires 28.8 mug NaIO4 and 2.5 mg KI per determination. Precise results (R.S.D. < 2%) in agreement with HPLC are obtained.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6