Spectrophotometric determination of phytic acid in plant extracts using a multi-pumping flow system

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Carneiro, JMT (Autor) Outra; Zagatto, EAG (Autor) Outra; Santos, JLM (Autor) FFUP; Lima, JLFC (Autor) FFUP

Revista

Título: Analytica Chimica Acta

Vol. 474 Nº 1

Páginas: 161-166

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 28 Citações

ISI Web of Science

Scopus - 35 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-000-M2Q

DOI: 10.1016/s0003-2670(02)01008-5

Resumo (PT): A flow-based spectrophotometric determination of phytic acid (PA) in foodstuffs involving PA extraction and reaction with Fe(III)-salicylate is proposed. Extraction is accomplished in a parallel array of ion-exchange mini-columns and the extracts are introduced into an improved flow system with multi-pumping. About 150 samples are run per hour, meaning 1.3×10−2 μg FeCl3·6H2O and 7.7×10−3 μg sodium salicylate per determination. Baseline drift is usually <0.01 absorbance per hour and the analytical signals for 5–100 mg l−1 PA range from 0.45 to 0.04 absorbance. Linearity of the analytical curve is fair (r>0.999, n=5) and detection limit was estimated as 1.0 mg l−1 PA. Results are precise (R.S.D.<1%) and in agreement with HPLC. <br> Keywords: Phytic acid; Multi-pumping; Flow analysis; Spectrophotometry; Food analysis <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TF4-4712KJ6-4&_user=2460038&_coverDate=12%2F09%2F2002&_rdoc=18&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235216%232002%23995259998%23360757%23FLA%23display%23Volume)&_cdi=5216&_sort=d&_docanchor=&_ct=23&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=569c46e0af708d363f4e6667fa8ef85d">Texto integral </a> <br> <br>

Abstract (EN): A flow-based spectrophotometric determination of phytic acid (PA) in foodstuffs involving PA extraction and reaction with Fe(III)-salicylate is proposed. Extraction is accomplished in a parallel array of ion-exchange mini-columns and the extracts are introduced into an improved flow system with multi-pumping. About 150 samples are run per hour, meaning 1.3 x 10(-2) mug FeCl3.6H(2)O and 7.7 x 10(-3) mug sodium salicylate per determination. Baseline drift is usually <0.01 absorbance per hour and the analytical signals for 5-100 mg l(-1) PA range from 0.45 to 0.04 absorbance. Linearity of the analytical curve is fair (r > 0.999, n = 5) and detection limit was estimated as 1.0 mg l(-1) PA. Results are precise (R.S.D. < 1%) and in agreement with HPLC.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6