LC-MS identification of derivatized free fatty acids from adipocere in soil samples

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Manuel Algarra (Autor) Outra; Jose E Rodriguez Borges (Autor) FCUP; Joaquim C G E Esteves da Silva (Autor) FCUP

Revista

Título: Journal of Separation Science

Vol. 33 Nº 20

Páginas: 143-154

ISSN: 1615-9306

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 17 Citações

Scopus - 18 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-003-9G5

DOI: 10.1002/jssc.200900614

Abstract (EN): Free fatty acids were derivatized as amides (DFFA) by reaction with (R)-(+)-1-phenyl-ethylamine, using a simple, fast and robust reaction scheme. A HPLC method with diode array and ESI MS detection was developed for the analysis of the derivatized substances. Six fatty acids were used in the method development: myristic, linoleic, palmitic, oleic, margaric and stearic acids. Under these conditions the elution of the DFFA are well resolved with retention times raging from 6.9 to 16.0 min. Fatty acids were extracted from cemetery soil and from adipocere formation experimental soils using a Soxhlet extraction, using as solvent ether/dichloromethane (1:1). Each DFFA is characterized by three m/z peaks: molecular weight of the substance; molecular weight of a dimer of the substance; the molecular weight of the dimer plus the atomic mass of sodium. The analysis of soil samples detected the six fatty acids used in the method developed plus palmitoleic and pentadecanoic. Beside this set of eight fatty acids other 13 fatty acids were detected in trace quantities or only in some soils and some were tentatively assigned as: 10-hydroxystearic, myristoleic, heptadecenoic and arachidic acids.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12