Alkaline phosphatase dual-binding sites for collagen dictate cell migration and microvessel assembly in vitro

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Susana Guerreiro (Autor) FCNAUP; Unger, RE (Autor) Outra; Cerqueira, NMFSA (Autor) Outra; Sartoris, A (Autor) Outra; Martins, MJ (Autor) FMUP; Barbosa, MA (Autor) Outra; Soares, R (Autor) Outra; Granja, PL (Autor) Outra; Kirkpatrick, CJ (Autor) Outra

Revista

Título: Journal of Cellular Biochemistry

ISSN: 0730-2312

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 4 Citações

Classificação Científica

FOS: Ciências médicas e da saúde

CORDIS: Ciências da Saúde

Outras Informações

ID Authenticus: P-00S-HKC

DOI: 10.1002/jcb.29835

Abstract (EN): Interactions between cell types, growth factors, and extracellular matrix components involved in angiogenesis are crucial for new vessel formation leading to tissue regeneration. This study investigated whether cocultures of fibroblasts and endothelial cells (ECs; from macro- or microvasculature) play a role in the formation of microvessel-like structures by ECs, as well as modulate fibroblast differentiation and growth factors production (vascular endothelial cell growth factor, basic fibroblast growth factor, active transforming growth factor-beta 1, and interleukin-8), which are important for vessel sprouting and maturation. Data obtained revealed that in vitro coculture systems of fibroblasts and human ECs stimulate collagen synthesis and growth factors production by fibroblasts that ultimately affect the formation and distribution of microvessel-like structures in cell cultures. In this study, areas with activated fibroblasts and high alkaline phosphatase (ALP) activity were also observed in cocultures. Molecular docking assays revealed that ALP has two binding positions for collagen, suggesting its impact in collagen proteins' aggregation, cell migration, and microvessel assembly. These findings indicate that bioinformatics and coculture systems are complementary tools for investigating the participation of proteins, like collagen and ALP in angiogenesis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14