Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Piloto, AML (Autor) Outra; Ribeiro, DSM (Autor) Outra; Rodrigues, SSM (Autor) Outra; Joao L M Santos (Autor) FFUP; Ferreira Sales, MGF (Autor) Outra

Revista

Título: Sensors and Actuators, B: Chemical

Vol. 304

ISSN: 0925-4005

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 7 Citações

Outras Informações

ID Authenticus: P-00R-EA4

DOI: 10.1016/j.snb.2019.127343

Abstract (EN): Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularlyimprinted technology (MIT), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N '-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4-19.2 pg/ml). Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12