Development of headspace solid-phase microextraction-gas chromatography-mass spectrometry methodology for analysis of terpenoids in Madeira wines

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2006

Autores

Camara, JS (Autor) Outra; Alves, MA (Autor) FEUP; Marques, JC (Autor) Outra

Revista

Título: Analytica Chimica Acta

Vol. 555 Nº 2

Páginas: 191-200

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 82 Citações

Scopus - 106 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-004-NPM

DOI: 10.1016/j.aca.2005.09.001

Abstract (EN): A dynamic headspace solid-phase microextraction methodology was developed for analysis of varietal aroma compounds in must and Madeira wine samples, a spirit wine with an ethanol content of 18% (v/v). The factors with influence in the headspace solid-phase microextraction efficiency such as: fibre coating, extraction time and temperature, pH, ionic strength, ethanol content, desorption time and temperature, were optimised and the method validated. The best results were obtained for a 85 Run polyacrylate fibre, with a 60 min headspace for must and 120 min for wine samples, in a 2.4 ml sample at 40 degrees C with 30% of NaCl. The extract is injected in the splitless mode in a GC-MS Varian system, Saturn III, and separated on a Stabilwax capillary column. The linear dynamic range of the method covers the normal range of occurrence of analytes in wine with typical r(2) between 0.985 (beta-ionone) and 0.998 (linalool) for musts and between 0.980 (alpha-terpineol) and 0.999 (linalool) for must and wine samples, respectively. For must samples the reproducibility ranges from 2.5% (citronellol) to 14.4% (nerolidol) (as R.S.D.), and from 4.8% (citronellol) to 14.2% (neiolidol) for wine samples. The analysis of spiked samples has shown that matrix effects do not significantly affect method performance. Limits of detection obtained are in low mu g I-1 range for all compounds analysed in this study.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10