Liposomes as a Model for the Biological Membrane: Studies on Daunorubicin Bilayer Interaction

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Carla Matos (Autor) Outra; Carla Moutinho (Autor) Outra; Paulo Lobao (Autor) FFUP

Revista

Título: Journal of Membrane Biology

Vol. 245

Páginas: 69-75

ISSN: 0022-2631

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 23 Citações

Scopus - 6 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

CORDIS: Ciências Naturais > Ciências biológicas > Biologia

Outras Informações

ID Authenticus: P-002-DB3

DOI: 10.1007/s00232-011-9414-2

Abstract (EN): In this study the interaction of the antitumoral drug daunorubicin with egg phosphatidylcholine (EPC) liposomes, used as a cell membrane model, was quantified by determination of the partition coefficient (K (p)). The liposome/aqueous-phase K (p) of daunorubicin was determined by derivative spectrophotometry and measurement of the zeta-potential. Mathematical models were used to fit the experimental data, enabling determination of K (p). In the partition of daunorubicin within the membrane both superficial electrostatic and inner hydrophobic interactions seem to be involved. The results are affected by the two types of interaction since spectrophotometry measures mainly hydrophobic interactions, while zeta-potential is affected by both interpenetration of amphiphilic charged molecules in the bilayer and superficial electrostatic interaction. Moreover, the degree of the partition of daunorubicin with the membrane changes with the drug concentration, due mainly to saturation factors. Derivative spectrophotometry and zeta-potential variation results, together with the broad range of concentrations studied, revealed the different types of interactions involved. The mathematical formalism applied also allowed quantification of the number of lipid molecules associated with one drug molecule.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7