Star-shaped magnetite@gold nanoparticles for protein magnetic separation and SERS detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Pedro Quaresma (Autor) Outra; Ines Osorio (Autor) Outra; Goncalo Doria (Autor) Outra; Patricia A Carvalho (Autor) Outra; Andre Pereira (Autor) FCUP; Judith Langer (Autor) Outra; Joao Pedro Araujo (Autor) FCUP; Isabel Pastoriza Santos (Autor) Outra; Luis M Liz Marzan (Autor) Outra; Ricardo Franco (Autor) Outra; Pedro V Baptista (Autor) Outra; Eulalia Pereira (Autor) FCUP

Revista

Título: RSC Advances

Vol. 4

Páginas: 3659-3667

ISSN: 2046-2069

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 87 Citações

Scopus - 94 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-008-K26

DOI: 10.1039/c3ra46762g

Abstract (EN): A novel synthetic methodology for star shaped gold-coated magnetic nanoparticles is reported. The coating is performed in two steps: formation of gold nuclei at the surface of magnetite nanoparticles followed by growth of the gold nuclei into a complete star shaped shell. The star-shaped gold-coated magnetic nanoparticles thus obtained preserve the magnetic properties of the precursor magnetite nanoparticles, e. g. they can be easily separated with a magnet. In addition, the gold coating provides interesting optical properties while simultaneously allowing for biofunctionalization that may be advantageous for biological applications, such as (bio)detection via surface-enhanced Raman spectroscopy (SERS). As a proof-of-concept, a capping agent terminated with a nickel(II)-nitrilotriacetate group showing high affinity for histidine was used to modify the surface of the nanoparticles. The resulting star-shaped nanoparticles were used to selectively capture histidine-tagged maltose-binding protein from a crude cell extract. Finally, the performance of star shaped gold-coated magnetic nanoparticles as SERS platforms was demonstrated through the detection of Raman active dye (Astra Blue).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9