Advances in protein solubility and thermodynamics: quantification, instrumentation, and perspectives

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Joana Ferreira (Autor) FEUP; Filipa Castro (Autor) Outra

Revista

Título: CrystEngComm

Vol. 25 Nº 46

Páginas: 6388-6404

ISSN: 1466-8033

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Projetos Associados

Healthy Waters - Healthy Waters - Identification, Elimination, Social Awareness and Education of Water Chemical and Biological Micropollutants with Health and Environmental Implications

Outras Informações

ID Authenticus: P-00Z-BFC

DOI: 10.1039/d3ce00757j

Abstract (EN): While protein crystallization has broadly been applied to determine the 3D structure of proteins, it has recently drawn great attention to replace the traditional downstream processing for protein-based biopharmaceuticals due to its advantages in stability, storage, and delivery. However, establishing the crystallization conditions of a protein remains a challenge because of the limited understanding of the underlying phenomena. This highlight provides a critical review of the advanced experimental approaches to measure thermodynamic parameters (e.g. solubility) that can help in establishing the necessary conditions to perform a protein crystallization trial. Firstly, methods and techniques to assess protein crystallizability and solution quality are presented. Next, methodologies to measure the main thermodynamic parameters are revised (with the respective advantages, limitations, and studied proteins). Later, protocols and set-ups (with a focus on microfluidic devices) used to quantify solubility parameters are highlighted (involved apparatus capabilities, solubility screening details, and studied proteins). Lastly, future directions and outlook of this critical review are approached by covering new trends in the research field. Critical review of the advanced experimental approaches to measure the main thermodynamic parameters and to guide establishing the necessary conditions to perform a protein crystallization assay: methodologies, set-ups, and future directions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17