Peroxynitrite and nitric oxide fluorescence sensing by ethylenediamine doped carbon dots

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Simoes, EFC (Autor) Outra; Joaquim C G E Esteves da Silva (Autor) FCUP; Leitao, JMM (Autor) Outra

Revista

Título: Sensors and Actuators, B: Chemical

Vol. 220

Páginas: 1043-1049

ISSN: 0925-4005

Editora: Elsevier

Indexação

ISI Web of Knowledge - 24 Citações

Scopus - 26 Citações

Outras Informações

ID Authenticus: P-00G-CKC

DOI: 10.1016/j.snb.2015.06.072

Abstract (EN): The selective fluorescence sensing of nitric oxide (NO) and peroxynitrite anion (ONOO-) in the presence of the principal reactive oxygen and nitrogen species (ROS/RNS) was achieved by fluorescence quenching through the pH variation of microwave synthesized fluorescent carbon dots (CDs) prepared from citric acid (CA) and ethylenediamine (EDA). The highest NO and ONOO- sensitivity, with the lowest sensitivity to the principal ROS/RNS, was demonstrated respectively at pH 4 and 10. The optimum synthesis and sensing conditions of CDs were obtained by multivariate full factorial experimental design methodologies: for NO, at pH 4, 2.5g of CA and 1000 mu L of EDA diluted in 15 mL of water exposed for 5 min to a microwave radiation of 700W; for ONOO-, at pH 7, 10-0.25 g of CA and 500 mu L of EDA diluted in 15 mL of water exposed for 5 min to a microwave radiation of 700W. In these optimized conditions the synthesized CDs have a quantum yield about 40%. The quantification of NO at pH 4 and of ONOO- at pH 7 and 10 were done in standard and in fortified serum solutions. Also the quantification of NO and of ONOO- in standard solutions was performed in the presence respectively of ONOO- and NO.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7