Official Code: | M802 |
Acronym: | M:BFBP |
Description: | O Mestrado em Biologia Funcional e Biotecnologia de Plantas é um programa de formação avançada focado essencialmente na biologia molecular e celular das plantas, com uma forte componente de I&D+i em áreas que vão da agroalimentar à medicina, passando pelo ambiente e farmacêutica. Este curso privilegia a aquisição de competências em ferramentas moleculares que permitem o desenvolvimento de aplicações biotecnológicas inovadoras, bem como o contacto dos estudantes com o tecido empresarial nesse setor, estimulando o empreendedorismo de base tecnológica e a transferência de conhecimento para a sociedade. |
The main objectives of this course are to provide students with ability to handle all bio-informatics tools available to the plant that is considered a model in molecular biology.
Familiarize themselves with advanced processing plants, handling of single and double null mutants and mutants with genes labeled with different tags. Employ techniques of emasculation and pollination of plants, studies semi in vivo of the pollen tube growth and visualization of molecular markers by fluorescence microscopy and electronic microscopy. Evaluation of results of Real Time-PCR and evaluating the results of RNA-seq.
Provide students with an understanding of tree growth; the connection between the structure of wood and the specific properties of wood products; a deep knowledge of the methods for wood identification; understanding of the importance of wood as natural resource and industrial material and how wood fits into different societies.
Most biologists contact with the problems of experimental design in a relaxed manner or not at all, refining their experiments empirically on a trial-error basis. During this course special attention will be paid to the logic underlying the whole process of experimental design, from drawing hypothesis, selection of statistical tests, planning and setup of experiments that unambiguously lead to interpretable results.
Aims of the course unit include are i) to provide advanced concepts in DNA manipulation, recombinant DNA technology, cloning and their applications in genetic transformation of plants and PCR, with special emphasis on the molecular techniques involved in the production of recombinant proteins; ii) to provide training in the use of basic bioinformatic tools for a protein expression project, primer design and gene optimization; iii) to provide laboratory “hands-on” training in gene cloning in expression vectors containing different tags and fusion proteins and transformation into appropriate bacterial strains.
The main objective of the course is that the students acquire solid knowledge about the physiological and biochemical processes underlying the damage caused by abiotic, namely organic pollutants, and biotic stresses and on the responses of acclimatization and adaptation of plants to these stressful situations, as well as identify strategies to increase plant tolerance to different types of stress.
Upon conclusion of the course, the student should be able to:
- understand aspects of plant physiology, more directly involved in the interactions plant-environment
- recognize the influence of climate and soil in plants
- explain how environmental factors can be responsible for severe stress situations
- recognize the main plant responses and adaptations to different stress situations
- know the main physiological and biochemical mechanisms that protect the plants against abiotic and biotic stresses
- understand the importance of knowledge in the field of stress physiology for solving environmental problems in the current context of climate change
With this UC we intend to provide the UC students with solid knowledge about the potential of aromatic and medicinal plants (PAM) for obtaining high-value-added products by biotechnological processes. For this goal the following specific objectives are outlined:
(a) Perception of the defense mechanisms of plants against adverse abiotic and biotic environmental factors based on secondary metabolites;
(b) Perception of the applications of secondary metabolites based on the respective biological activities;
(c) Understanding the capabilities and limitations of production of secondary metabolites of high economic value by cell cultures, plant tissues and organs;
(d) Understanding of various techniques and methodologies that involve cultures of aromatic and medicinal plants for biotechnological production of compounds of high commercial value, such as:
d.1 - cultures in solid and in liquid medium in batch system and in continuous system, using bioreactors;
d.2 - elicitation techniques;
d.3 - techniques involving recombinant DNA;
d.4 - immobilization processes of molecules, cells, meristems and somatic embryos
d.5 - artificial seed production processes;
d.6 - processes of conservation/cryopreservation.
The aim of this course is the understanding of signal transduction pathways in plants that coordinate gene expression with the development and the responses of plants to environmental conditions, providing the basis for regulation of physiology and development ie its Functional Biology.
In this UC is also intended that students come into contact with the investigation conducted in UP in the area of Biotechnology and Functional Biology of Plants, guiding them in the choice of dissertation theme.
After completion of curricular unit, the student should be able to:
- Understand the general principles of signal transduction in plants
- Recognize the signaling pathways involved in some physiological processes that occur during development or in response to environmental factors
- Understand the interaction between different signaling pathways
- Know methodologies and technology used in research in the area of Functional Biology of Plants.Principal aims: To develop an integrated view of the structure and function of genomes, transcriptomes and proteomes and to know the modern methodologies associated with functional genomics, including sequencing, annotation, in silico analysis of functional genomics databases, transcriptomics and main principles of bioinformatics.
Specific aims:
-Compare the genes and / or gene family among model plants like Arabidopsis thaliana and species of agronomic interest; understand comparative genomic resources.
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- To use the main in silico/bioinformatics tools for the characterization of genes and proteins in plants.
-Analyze RNA-seq gene expression data towards the characterization of differentially expressed genes.
-To analyze proteomics data, in order to characterize proteins with regards to: biochemical parameters, prediction of subcellular localization and post-translational modifications - phosphorylation and glicosilation; 3D structure prediction.
-Know fundamental principles of bioinformatics analysis; experiment on the use of command lines in a LINUX environment.
The UC aims to develop in the students skills in the creation of new business ventures in the area of life sciences, integrating both a theoretical and a strong practical component. The presentation and discussion of concepts and methodologies, will aim to validate the application of the market potential of a concrete business project in the area of biosciences.
At the end of the course, students should:
1 Understanding the challenges of the creation, validation and start-up of new business process projects;
Provide students with basic knowledge of in vitro plant cell cultures and practical applications, including micropropagation and transformation of plants. Provide students with basic knowledge of manipulation in aseptic conditions.
During a normal period of one year, and as a basic part of their training, students should develop an original research project in the field of fundamental or applied Biotechnology and functional Biology of Plants. This work will lead to the drafting of a final dissertation that will form the keystone of the evaluation of the student. This work should still result in publications in international journals with peer review system.
Alternatively the student may perform an internship and prepare a final report which will form the basis of their evaluation.During a normal period of one year, and as a basic part of their training, students should develop an original research project in the field of fundamental or applied Biotechnology and functional Biology of Plants in a comapny context. This work will lead to the drafting of a final report that will form the keystone of the evaluation of the student. This work may still result in publications in international journals with peer review system.